The use of flow cytometry to evaluate temporal changes in inflammatory cells following focal cerebral ischemia in mice
The use of flow cytometry to evaluate temporal changes in inflammatory cells following focal cerebral ischemia in mice
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DOI：
10.1016/s0006-8993(02)02292-8
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发表时间：
2002-04-05
期刊：
影响因子：
2.9
通讯作者：
Stenzel-Poore, MP
中科院分区：
文献类型：
--
作者：
Stevens, SL;Bao, JZ;Stenzel-Poore, MP
最近的研究表明，脑缺血后的炎症反应有助于神经元损伤。驻留细胞的局部活化和白细胞进入中枢神经系统的有效募集是该炎症过程中的关键步骤。在这里，我们描述了使用流式细胞术研究小鼠短暂大脑中动脉闭塞（MCAO）后炎症细胞活化和浸润的时间模式。我们发现早在闭塞后18小时活化的小胶质细胞/巨噬细胞增加，其在闭塞后48小时达到峰值并在闭塞后96小时保持丰富。中性粒细胞在48小时显著增加，并在闭塞后96小时保持升高。T淋巴细胞增加相对较晚（72和96小时）闭塞后。流式细胞术数据与在相同小鼠上进行的免疫组织化学分析在定量和定性上都很好地相关。本研究证明了流式细胞术在分析脑缺血后炎症过程中的作用，并提供了短暂MCAO后小鼠细胞变化的时间信息。(C)2002 Elsevier Science B. V.保留所有权利。
Recent studies indicate that inflammation following cerebral ischemia contributes to neuronal damage. The local activation of resident cells and efficient recruitment of leukocytes into the central nervous system are critical steps in this inflammatory process. Here we describe studies using flow cytometry to examine the temporal pattern of inflammatory cell activation and infiltration following transient middle cerebral artery occlusion (MCAO) in mice. We found an increase in activated microglia/macrophages as early as 18 h post Occlusion, which peaked at 48 h and remained abundant at 96 It post occlusion. Neutrophils were significantly increased by 48 h and remained elevated at 96 h post occlusion. T lymphocytes were increased relatively late (72 and 96 h) post occlusion. The flow cytometry data correlate well both quantitatively and qualitatively with immunohistochemistry analysis performed on the same mice. The present study demonstrates the power of flow cytometry in analyzing the inflammatory process following cerebral ischemia and offers temporal information on the Cellular changes in mice following transient MCAO. (C) 2002 Elsevier Science B.V. All rights reserved.