PRD1, a homologous recombination initiation factor, is involved in spindle assembly in rice meiosis.
PRD1, a homologous recombination initiation factor, is involved in spindle assembly in rice meiosis.
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DOI：
10.1111/nph.17178
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发表时间：
2021-01
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影响因子：
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通讯作者：
Wenqing Shi;Jianhui Ji;Z. Xue;Fanfan Zhang;Yongjie Miao;Han Yang;Ding Tang;Guijie Du;Yafei Li;Yi Shen;Zhukuan Cheng
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作者：
Wenqing Shi;Jianhui Ji;Z. Xue;Fanfan Zhang;Yongjie Miao;Han Yang;Ding Tang;Guijie Du;Yafei Li;Yi Shen;Zhukuan Cheng
减数分裂过程中的双极性纺锤体结构对染色体的忠实分离至关重要。推定的重组起始缺陷1 （PRD1）先前已被证明参与DNA双链断裂（dsb）的形成。然而，PRD1在减数分裂纺锤体组装中的作用尚未阐明。本研究通过遗传分析和免疫染色技术发现，PRD1参与了水稻减数分裂的纺锤体组装。我们发现DSB的形成和双极纺锤体组装在prd1减数细胞中受到干扰。PRD1信号显示了一个动态的定位模式，从覆盖整个染色体在瘦素后聚集在着丝粒区域。PRD1信号的着丝定位取决于瘦素染色体的组织，而不取决于DSB的形成和轴的建立。PRD1与几个着丝点组分相互作用和共定位。我们还发现，在REC8突变诱导的单价中，姐妹着丝点的双取向可以恢复prd1的双极性。此外，PRD1直接与REC8和SGO1相互作用，表明PRD1可能在调节姐妹着丝点的定向中发挥作用。综上所述，我们推测PRD1可能通过调节水稻减数分裂中姐妹着丝点的方向来促进双极纺锤体组装。
The bipolar spindle structure in meiosis is essential for faithful chromosome segregation. PUTATIVE RECOMBINATION INITIATION DEFECT 1 (PRD1) has been previously shown to participate in the formation of DNA double strand breaks (DSBs). However, the role of PRD1 in meiotic spindle assembly has not been elucidated. Here, we reveal by both genetic analysis and immunostaining technology that PRD1 is involved in spindle assembly in rice (Oryza sativa) meiosis. We show that DSB formation and bipolar spindle assembly are disturbed in prd1 meiocytes. PRD1 signals display a dynamic pattern of localization from covering entire chromosomes at leptotene to congregating at the centromere region after leptotene. Centromeric localization of PRD1 signals depends on the organization of leptotene chromosomes, but not on DSB formation and axis establishment. PRD1 exhibits interaction and co-localization with several kinetochore components. We also find bi-orientation of sister kinetochores within a univalent induced by mutation of REC8 can restore bipolarity in prd1. Furthermore, PRD1 directly interacts with REC8 and SGO1, suggesting that PRD1 may play a role in regulating the orientation of sister kinetochores. Taken together, we speculate that PRD1 promotes bipolar spindle assembly, presumably by modulating the orientation of sister kinetochores in rice meiosis.