A NMR study of parasitized tenebrio molitor and Hymenolepis diminuta cysticercoids
A NMR study of parasitized tenebrio molitor and Hymenolepis diminuta cysticercoids
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DOI：
10.1016/0020-7519(96)00050-1
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发表时间：
1996-07-01
影响因子：
4
通讯作者：
Blackburn, BJ
中科院分区：
文献类型：
--
作者：
Schoen, J;Modha, A;Blackburn, BJ
舍恩·J Modha A.，马斯洛，诺瓦克·M & Blackburn B. J. 1996年。寄生黄粉虫和缩小膜壳绦虫囊尾蚴的核磁共振研究International Journal for Parasitology 26：713-722.在体内NMR光谱的未感染和Hymenolepis diminuta感染黄粉虫喂养D-（1-C-13）葡萄糖显示，感染的甲虫的两种性别有显着较高的比例（糖原C-1/脂质（CH 2）（n））比相应的控制。定量代谢：根据高氯酸提取物的MMR，列出了对照和饲喂D-（1-C-13）葡萄糖的感染甲虫以及H.类囊尾蚴雌性甲虫，控制和感染，含有更多的糖原比他们的男性同行和感染的甲虫的两种性别具有较少的甘油磷酸胆碱，但更多的糖原和更高的百分比标签葡萄糖和海藻糖比各自的控制。标记物还被掺入糖原、琥珀酸、乙酸、丙氨酸和乳酸中。从饲喂D-（1-C-13）葡萄糖的甲虫中提取的囊尾蚴含有以下标记化合物，按C-13标记百分比递减的顺序：葡萄糖、海藻糖、丙氨酸、琥珀酸盐、乳酸盐、糖原和乙酸盐。采用D-（1-C-13）葡萄糖的体外培养实验表明，在囊尾蚴中发现的海藻糖是寄生虫而不是甲虫来源的。版权所有（C）1996澳大利亚寄生虫学会。
Schoen J., Modha A., Maslow K., Novak M. & Blackburn B. J. 1996. A NMR study of parasitized Tenebrio molitor and Hymenolepis diminuta cysticercoids. International Journal for Parasitology 26: 713-722. In vivo NMR spectra of uninfected and Hymenolepis diminuta-infected Tenebrio molitor fed D-(1-C-13)glucose showed that infected beetles of both sexes had a significantly higher ratio for (glycogen C-1/lipid (CH2)(n)) than the corresponding controls. Quantitative metabolic: profiles and the per cent C-13-label in metabolites, based on MMR of perchloric acid extracts, are presented for control and infected beetles fed D-(1-C-13)glucose and for H. diminuta cysticercoids. Female beetles, both control and infected, contained more glycogen than their male counterparts and infected beetles of both sexes possessed less glycerophosphocholine, but more glycogen and a higher percentage label in glucose and trehalose than their respective controls. Label was also incorporated into glycogen, succinate, acetate, alanine and lactate. Extracts of cysticercoids from beetles fed D-(1-C-13)glucose contained the following labelled compounds, in order of decreasing per cent C-13 label: glucose, trehalose, alanine, succinate, lactate, glycogen and acetate, In vitro cultivation experiments, employing D-(1-C-13)glucose, revealed that trehalose found in cysticercoids was of parasite, and not beetle, origin. Copyright (C) 1996 Australian Society for Parasitology.