In vivo chitin-cadmium complexation in cell wall of Neurospora crassa
In vivo chitin-cadmium complexation in cell wall of Neurospora crassa
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DOI：
10.1016/s0304-4165(00)00090-8
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发表时间：
2000-09-01
影响因子：
3
通讯作者：
Venkateswerlu, G
中科院分区：
文献类型：
--
作者：
Bhanoori, M;Venkateswerlu, G
真菌细胞壁主要由几丁质（一种N-乙酰氨基葡萄糖聚合物）组成，参与重金属解毒。本研究试图阐明粗糙脉孢菌细胞壁几丁质物质与镉络合的位点。根据固体核磁共振、X射线衍射、红外光谱和分子模拟等物理技术的结果，推测了甲壳素-镉配合物的结构。N-乙酰葡萄糖胺的环和C-3羟基氧参与了真菌细胞壁几丁质对镉的络合作用。研究进一步表明，镉络合后甲壳素的构象没有发生变化。(C)2000 Elsevier Science B. V.保留所有权利。
Fungal cell wall, mainly composed of chitin, an N-acetylglucosamine polymer, is known to participate in heavy metal detoxification. In the present study, an effort was made to elucidate the sites involved in complexation of cadmium by the chitin material of cell wall of Neurospora crassa. Based on the results of physical techniques, such as solid-state C-13-NMR, X-ray diffraction, IR and molecular modeling, a structure was proposed for the chitin-cadmium complex. The ring and C-3 hydroxyl oxygens of N-acetylglucosamine were implicated in the complexation of cadmium by the chitin of the fungal cell wall. The studies further revealed that the conformation of chitin did not alter after cadmium complexation. (C) 2000 Elsevier Science B.V. All rights reserved.