Inhibitory effect of D-Tryptophan on the spoilage potential of Shewanella baltica and Pseudomonas fluorescens and its potential application in salmon fillet preservation
Inhibitory effect of D-Tryptophan on the spoilage potential of Shewanella baltica and Pseudomonas fluorescens and its potential application in salmon fillet preservation
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DOI：
10.1016/j.fm.2022.104104
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发表时间：
2022-12-01
影响因子：
5.3
通讯作者：
Yanbo
中科院分区：
文献类型：
--
作者：
Chen, Jian;Yang, Rendi;Yanbo
本研究发现D-色氨酸（D-Trp）对荧光假单胞菌（P. fluorescens）和S.波罗的海。D-Trp对细菌生长的抑制作用与环境中NaCl浓度密切相关。生长/无生长模型的开发，以澄清盐和D-Trp浓度之间的关系，以获得最佳的生长抑制效果。结果表明，生长抑制需要较高水平的NaCl（>3.5%）和D-Trp（>15 mM）。D-Trp与NaCl联合处理也抑制了低温下的嗜冷菌生长，减少了呼吸活性菌的数量，表明D-Trp的抑制作用可能与其对渗透胁迫和低温胁迫的耐受性有关。此外，研究了D-Trp与NaCl（人工海水）联合处理鲑鱼片的效果。在冷藏期间，D-Trp处理的鲑鱼片或者接种S. baltica或荧光假单胞菌保持相对低的总挥发性碱性氮（TVBN）水平（在8天的储存期间低于30 mg N/100 g），并且延迟了低的总活菌计数（TVC）的增加，延迟期为2天。
In this study, we found the inhibitory effect of D-Tryptophan (D-Trp) on the bacterial growth and spoilage potential of P. fluorescens and S. baltica. The effectiveness of D-Trp in bacterial growth inhibition was highly dependent on environmental NaCl concentrations. The growth/no growth models were developed to clarify the relationship between salt and D-Trp concentrations for optimal growth-inhibitory effect. It showed that higher levels of NaCl (>3.5%) with D-Trp (>15 mM) are required for growth inhibition. The D-Trp treatment combined with NaCl also retarded the psychrotrophic growth and reduced the respiratory active bacteria at low temperature, indicating that the inhibitory effect of D-Trp might be associated with the tolerance to osmotic and cold stress. Furthermore, the treatment D-Trp combined NaCl (artificial seawater) on salmon fillets was investigated. During refrigerated storage, the D-Trp treated salmon fillet either inoculated with S. baltica or P. fluorescen maintained remained a relatively low level of total volatile basic nitrogen (TVBN) (below 30 mg N/100 g during 8-days storage) and delayed increase of low total viable bacteria counts (TVC) with a longer lag phase of 2 days.