Protein glycation and oxidation inhibitory activity of Centella asiatica phenolics (CAP) in glucose-mediated bovine serum albumin glycoxidation
Protein glycation and oxidation inhibitory activity of Centella asiatica phenolics (CAP) in glucose-mediated bovine serum albumin glycoxidation
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DOI：
10.1016/j.foodchem.2020.127302
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发表时间：
2020-12-01
期刊：
影响因子：
8.8
通讯作者：
Tola, Adesola Julius
中科院分区：
文献类型：
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作者：
Eze, Fredrick Nwude;Tola, Adesola Julius
在体外牛血清白蛋白-葡萄糖模型上，观察积雪草酚类化合物(CAP)对牛血清白蛋白糖氧化的抑制作用。测定了酚类提取物对总荧光晚期糖基化终产物(AGEs)和Amadori加合物形成的影响。二核苷酸、N-甲酰基犬尿氨酸、犬尿氨酸和蛋白质羰基被定量作为蛋白质氧化的标志。通过刚果红结合和FTIR分析确定了蛋白质的结构扰动。采用比色法和UHPLC-ESI-qTOF-MS联用技术进行化学表征和CAP成分图谱分析。我们的数据显示，CAP减少了荧光AGEs(38.5%)、二核苷酸(44.6%)、N-甲酰基犬尿氨酸(42.9%)、Amadori产物的形成，并抵抗糖基化引起的BSA的结构变化。这些作用可能是由酚类和三萜类化合物介导的CAP的抗氧化和清除自由基活性所致。这些结果表明，CAP具有降血糖作用，具有潜在的营养保健应用前景。
The study aimed to evaluate the inhibitory effects of Centella asiatica phenolics (CAP) on bovine serum albumin glycoxidation in a BSA-glucose model in vitro. The impact of the phenolic extract on the formation of total fluorescent advanced glycation end products (AGEs) and Amadori adducts were determined. Dityrosine, N-formylkynurenine, kynurenine and protein-carbonyls were quantified as markers of protein oxidation. Protein structural perturbations were determined by Congo red binding and FTIR analysis. Chemical characterization and CAP phytoconstituent profile was obtained by colorimetric and UHPLC-ESI-qTOF-MS analysis, respectively. Our data show that CAP attenuated the formation of fluorescent AGEs (38.5%), Dityrosine (44.6%), N-formylkynurenine (42.9%), Amadori products, and resisted structural alterations of BSA subjected to glycation. These effects could be due to the antioxidant and radical scavenging activities of CAP mediated by the presence of phenolics and triterpenoids. The results collectively suggest that CAP possesses antiglycative properties with potentials for nutraceutical applications.