p40phox, a third cytosolic component of the activation complex of the NADPH oxidase to contain src homology 3 domains.
p40phox, a third cytosolic component of the activation complex of the NADPH oxidase to contain src homology 3 domains.
复制标题
p40phox，NADPH 氧化酶激活复合物的第三个胞质成分，包含 src 同源 3 结构域。
作者：
F. Wientjes;J. Hsuan;N. Totty;A. Segal
NADPH氧化酶在吞噬细胞中产生超氧化物。它对免疫很重要，其缺乏会导致慢性肉芽肿病（CGD）。它由膜结合的黄细胞色素B组成，该黄细胞色素处于休眠状态，直到被胞质蛋白p47 phox（吞噬细胞氧化酶的phox）、p67 phox和p21 rac（一种小的GTP结合蛋白）转运至质膜而激活。我们在这里表明，一个新的组件，p40 phox，形成一个激活复合物与p47 phox和p67 phox与它易位到膜与黄细胞色素B。cDNA克隆和氨基酸序列分析表明，p40 phox具有src同源性3（SH 3）结构域，与p47 phox的N-末端有很大的序列相似性。p40 phox的主要关联似乎是与p67 phox，它是目前在CGD缺乏p67 phox的患者减少量。
The NADPH oxidase generates superoxide in phagocytic cells. It is important for immunity and its deficiency leads to chronic granulomatous disease (CGD). It consists of a membrane-bound flavocytochrome b that lies dormant until activated by the translocation to the plasma membrane of cytosolic proteins, p47phox (phox for phagocyte oxidase), p67phox and p21rac, a small GTP-binding protein. We show here that a novel component, p40phox, forms an activation complex with p47phox and p67phox with which it translocates to the membrane to associate with the flavocytochrome b. cDNA cloning and amino acid analysis revealed that p40phox has an src homology 3 (SH3) domain and a large region of sequence similarity with the N-terminus of p47phox. The primary association of p40phox appears to be with p67phox, and it is present in reduced amounts in patients with CGD lacking p67phox.