Molecular Cytogenetic Analysis of the Scleractinian Coral Acropora solitaryensis Veron & Wallace 1984
Molecular Cytogenetic Analysis of the Scleractinian Coral Acropora solitaryensis Veron & Wallace 1984
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DOI：
10.2108/zsj.31.89
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发表时间：
2014-02-01
影响因子：
0.9
通讯作者：
Tominaga, Akira
中科院分区：
文献类型：
--
作者：
Taguchi, Takahiro;Mezaki, Takuma;Tominaga, Akira
对日本温带地区（北纬30-35度）占优势的核核珊瑚Acropora solitaryensis进行了分子细胞遗传学研究。分子细胞遗传学分析采用荧光原位杂交（FISH）进行核型分析和基因定位。我们根据c -band和FISH分析提出了这种珊瑚（2n = 30）的核型。rRNA基因的FISH定位使用rRNA基因引物通过PCR扩增产生的探针进行。此外，使用FISH可视化所有染色体的端粒和着丝粒。通过对该珊瑚的精子和未受精卵的DNA进行比较基因组杂交，我们提供了证据表明该物种存在性染色体。总的来说，这些数据促进了我们对珊瑚遗传学的理解。
We performed a molecular cytogenetic investigation of the scleractinian coral Acropora solitaryensis, which is dominant in the temperate region of Japan (30-35 degrees N). Molecular cytogenetic analysis, using fluorescence in situ hybridization (FISH), was carried out for karyotyping and gene mapping. We propose the karyotype of this coral (2n = 30) based on C-banding and FISH analyses. FISH mapping of the rRNA gene was carried out with a probe generated by PCR amplification using rRNA gene primers. Furthermore, the telomeres and centromeres of all chromosomes were visualized using FISH. By comparative genomic hybridization using DNA from sperm and unfertilized eggs of this coral, we offer evidence suggesting the existence of sex chromosomes in this species. Collectively, these data advance our understanding of coral genetics.