Membrane deformation of living glial cells using atomic force microscopy
Membrane deformation of living glial cells using atomic force microscopy
复制标题
DOI：
10.1046/j.1365-2818.1996.141423.x
复制
发表时间：
1996-05-01
期刊：
影响因子：
--
通讯作者：
MarcheseRagona, SP
中科院分区：
文献类型：
--
作者：
Haydon, PG;Lartius, R;MarcheseRagona, SP
使用原子力显微镜（AFM），尽管AFM针尖被施加到细胞表面，但已经可以检测活细胞的细胞膜下方的肌动蛋白丝。为了确定AFM针尖是否真正穿透细胞膜或使细胞膜变形，我们确定在AFM期间细胞内捕获的荧光指示剂是否从细胞中丢失。利用落射荧光照明来监测细胞中fluo-3的存在，我们发现AFM不会导致染料从细胞中泄漏。此外，力-距离曲线表明，标准针尖不穿透膜，而更锋利的Supertips（TM）穿透膜。此外，发现使用标准针尖的AFM不影响细胞的生理，因为在这些研究中体积调节信号转导机制是完整的。因此，传统的AFM针尖变形的细胞膜，以揭示亚细胞结构的存在。
Using atomic force microscopy (AFM) it has been possible to detect actin filaments that are beneath the cell membrane of living cells despite the fact that the AFM tip is applied to the surface of the cell. To determine whether the AFM tip actually penetrates or deforms the cell membrane we determined whether an intracellularly trapped fluorescent indicator was lost from cells during AFM. Using epifluorescence illumination to monitor the presence of fluo-3 in the cell, we found that AFM did not cause dye leakage from the cell, Further, force-distance curves indicated that standard tips did not penetrate the membrane while sharper Supertips(TM) did, In addition, the physiology of cells was found to be unaffected by AFM with standard tips since volume regulatory signal transduction mechanisms were intact in such studies. Thus, traditional AFM tips deform the cell membrane in order to reveal the presence of subcellular structures.