Paper based point-of-care testing disc for multiplex whole cell bacteria analysis
Paper based point-of-care testing disc for multiplex whole cell bacteria analysis
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DOI：
10.1016/j.bios.2011.04.035
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发表时间：
2011-07-15
影响因子：
12.6
通讯作者：
Almeide, Eugenio
中科院分区：
文献类型：
--
作者：
Li, Chen-zhong;Vandenberg, Katherine;Almeide, Eugenio
感染性细菌病原体的护理点检测（POCT）为疾病诊断提供了实质性的好处，主要是通过缩短获得结果所需的时间，以及通过在床边或远程护理中心提供检测。免疫层析侧流生物传感器为POCT提供了一个低成本、高灵敏度的平台。在这篇文章中，我们描述了一个多重免疫圆盘传感器的制作和测试的铜绿假单胞菌和金黄色葡萄球菌的特异性检测。抗体结合的金纳米颗粒被用作信号传导剂。细菌的检测范围为500-5000 CFU/ml。免疫圆盘传感器的优点是它不需要对生物样品进行任何预处理，并且能够进行全细胞细菌检测。我们还描述了一个紧凑的便携式设备的设计和制造，它转换成定量的电压阅读成比例的样品中的细菌浓度的测试区域积累的金纳米粒子的颜色强度。免疫盘和便携式颜色读取器的组合提供了用于检测患者样品中存在的一组感染因子的快速、灵敏、低成本和定量工具。(C)2011 Elsevier B. V.保留所有权利。
Point-of-care testing (POCT) of infectious bacterial agents offers substantial benefits for disease diagnosis, mainly by shortening the time required to obtain results and by making the test available bedside or at remote care centers. Immunochromatographic lateral flow biosensors offer a low cost, highly sensitive platform for POCT. In this article, we describe the fabrication and testing of a multiplex immuno-disc sensor for the specific detection of Pseudomonas aeruginosa and Staphylococcus aureus. Antibody conjugated gold nanoparticles were used as the signaling agents. The detection range of the bacteria lies within 500-5000 CFU/ml. The advantage of the immuno-disc sensor is that it does not require any preprocessing of biological sample and is capable of whole cell bacterial detection. We also describe the design and fabrication of a compact portable device which converts the color intensity of the gold nanoparticles that accumulate at the test region into a quantitative voltage reading proportional to the bacterial concentration in the sample. The combination of the immuno-disc and the portable color reader provides a rapid, sensitive, low cost, and quantitative tool for the detection of a panel of infectious agents present in the patient sample. (C) 2011 Elsevier B.V. All rights reserved.