Age, absolute CD4 count, and CD4 percentage in relation to HPV infection and the stage of cervical disease in HIV-1-positive women
Age, absolute CD4 count, and CD4 percentage in relation to HPV infection and the stage of cervical disease in HIV-1-positive women
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DOI：
10.1097/md.0000000000019273
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发表时间：
2020-02-01
期刊：
影响因子：
1.6
通讯作者：
Gray, Clive M.
中科院分区：
文献类型：
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作者：
Chambuso, Ramadhani;Ramesar, Raj;Gray, Clive M.
合并感染 1 型人类免疫缺陷病毒 (HIV-1) 和人乳头瘤病毒 (HPV) 的女性亚群会迅速发展为宫颈疾病。我们对宫颈肿瘤活检中的 HPV 基因型进行了表征，评估了宫颈疾病分期与年龄、HIV-1 状态、绝对 CD4 计数和 CD4 百分比的关系，并确定了这些变量对南非女性队列中宫颈疾病分期的预测能力。我们招募了 181 名经组织学诊断患有宫颈疾病的女性； 87 人为 HIV-1 阳性，94 人为 HIV-1 血清阴性。阴道镜引导的肿瘤活检经过组织学证实并用于基因组 DNA 提取。 HPV基因分型采用罗氏线性阵列HPV基因分型测试。外周全血用于 HIV-1 快速检测。使用带有 PanLeucogate (PLG) 的全自动 FC500MPL/CellMek 来确定绝对 CD4 计数、CD4 百分比和 CD45 计数。卡方检验、逻辑回归模型、参数皮尔逊相关和ROC曲线用于统计分析。我们使用 Benjamini-Horchberg 检验来控制错误发现率（FDR，q 值）。当 P 和 q 均满足时，所有检验均显着
A subgroup of women who are co-infected with human immunodeficiency virus type 1 (HIV-1) and human papillomavirus (HPV), progress rapidly to cervical disease. We characterized HPV genotypes within cervical tumor biopsies, assessed the relationships of cervical disease stage with age, HIV-1 status, absolute CD4 count, and CD4 percentage, and identified the predictive power of these variables for cervical disease stage in a cohort of South African women.We recruited 181 women who were histologically diagnosed with cervical disease; 87 were HIV-1-positive and 94 were HIV-1seronegative. Colposcopy-directed tumor biopsies were confirmed by histology and used for genomic DNA extraction. The Roche Linear Array HPV genotyping test was used for HPV genotyping. Peripheral whole blood was used for HIV-1 rapid testing. Fully automated FC500MPL/CellMek with PanLeucogate (PLG) was used to determine absolute CD4 count, CD4 percentage, and CD45 count. Chi-squared test, a logistic regression model, parametric Pearson correlation, and ROC curves were used for statistical analyses. We used the Benjamini-Horchberg test to control for false discovery rate (FDR, q-value). All tests were significant when both P and q were