Identification of Potential Genes for Benign Prostatic Hyperplasia and Prostate Cancer Susceptibility in Four X-chromosome Regions with High Frequency of Microvariant Alleles.
Identification of Potential Genes for Benign Prostatic Hyperplasia and Prostate Cancer Susceptibility in Four X-chromosome Regions with High Frequency of Microvariant Alleles.
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DOI：
10.31557/apjcp.2020.21.8.2271
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发表时间：
2020-08-01
期刊：
影响因子：
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通讯作者：
Ismail P
中科院分区：
文献类型：
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作者：
Albujja MH;Messaudi SA;Vasudevan R;Al Ghamdi S;Chong PP;Ghani KA;Ranneh Y;Alaidarous M;Ismail P
X染色体被认为在前列腺癌（PrCa）中发挥作用，因为流行病学研究提供了证据，证明PrCa的X连锁遗传模式，其基础是与报告受影响父亲的男性相比，报告受影响兄弟的男性的相对风险更高。本研究的目的是检查位于Xp22.31，Xq11.2-12，Xq26.2和Xq 28四个区域的法医STR标记与BPH和PrCa风险之间的潜在关联，以确认ChrX在PrCa发病率中的影响。这可能有助于将STR遗传变异纳入早期检测男性人群中的PrCa风险。 使用Investigator® Argus X-12 QS试剂盒分析了从沙特阿拉伯利雅得的两个医学中心采集的92名患者和156名健康对照的DNA样本中位于X染色体的四个区域。 结果表明，微变异等位基因（DXS 7132、DXS 10146、HPRTB、DXS 10134和DXS 10135）在BPH组中的代表性过高（p < 0.00001）。DXS 10135的等位基因28和DXS 7423的等位基因15可能具有保护作用，OR值分别为0.229（95%CI，0.066-0.79）和0.439（95%CI，0.208-0.925）。另一方面，携带DXS 10079等位基因23和DXS 10148等位基因26的患者出现PrCa风险增加OR 4.714（95%CI，3.604-6.166）。 结果与X染色体参与PrCa和BPH的发展是一致的。STR等位基因研究可以从PrCa抗性或易感性的遗传特征的定义中添加进一步的信息。由于TBL 1、AR、LDOC 1和RPL 10基因分别位于Xp22.31、Xq11.2-12、Xq26.2和Xq 28区域，因此这些基因可能在PrCa或BPH中起重要作用。
The X-chromosome has been suggested to play a role in prostate cancer (PrCa) since epidemiological studies have provided evidence for an X-linked mode of inheritance for PrCa based on the higher relative risk among men who report an affected brother(s) as compared to those reporting an affected father. The aim of this study was to examine the potential association between the forensic STR markers located at four regions Xp22.31, Xq11.2-12, Xq26.2, and Xq28 and the risk of BPH and PrCa to confirm the impact of ChrX in the PrCa incidence. This may be helpful in the incorporation of STRs genetic variation in the early detection of men population at risk of developing PrCa. DNA samples from 92 patients and 156 healthy controls collected from two medical centers in Riyadh, Saudi Arabia were analyzed for four regions located at X-chromosome using the Investigator® Argus X-12 QS Kit. The results demonstrated that microvariant alleles of (DXS7132, DXS10146, HPRTB, DXS10134, and DXS10135) are overrepresented in the BPH group (p < 0.00001). Allele 28 of DXS10135 and allele 15 of DXS7423 could have a protective effect, OR 0.229 (95%CI, 0.066-0.79); and OR 0.439 (95%CI, 0.208-0.925). On the other hand, patients carrying allele 23 of DXS10079 and allele 26 of DXS10148 presented an increased risk to PrCa OR 4.714 (95%CI, 3.604-6.166). The results are in concordance with the involvement of the X chromosome in PrCa and BPH development. STR allele studies may add further information from the definition of a genetic profile of PrCa resistance or susceptibility. As TBL1, AR, LDOC1, and RPL10 genes are located at regions Xp22.31, Xq11.2-12, Xq26.2, and Xq28, respectively, these genes could play an essential role in PrCa or BPH.