Achieving increased resolution and more pixels with Superresolution Optical Fluctuation Imaging (SOFI).
Achieving increased resolution and more pixels with Superresolution Optical Fluctuation Imaging (SOFI).
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DOI：
10.1364/oe.18.018875
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发表时间：
2010-08-30
期刊：
影响因子：
3.8
通讯作者：
Weiss S
中科院分区：
文献类型：
--
作者：
Dertinger T;Colyer R;Vogel R;Enderlein J;Weiss S
最初证明的超分辨率光学波动成像（SOFI）可以将成像分辨率增强两倍的平方根。在这里，我们演示了如何通过重新加权光学传递函数（OTF）来提高SOFI图像的分辨率。此外，我们演示了如何交叉累积量可以利用，以获得一个公平的近似的基础点扩散函数。我们显示了近红外量子点标记的3T3成纤维细胞微管蛋白网络的分辨率（超过衍射极限）的两倍增加
Superresolution Optical Fluctuation Imaging (SOFI) as initially demonstrated allows for a resolution enhancement in imaging by a factor of square-root of two. Here, we demonstrate how to increase the resolution of SOFI images by re-weighting the Optical Transfer Function (OTF). Furthermore, we demonstrate how cross-cumulants can be exploited to obtain a fair approximation of the underlying Point-Spread Function. We show a two-fold increase of resolution (over the diffraction limit) of near-infrared quantum dot labeled tubulin-network of 3T3 fibroblasts