Enhancement of bone formation ex vivo and in vivo by a helioxanthin-derivative
Enhancement of bone formation ex vivo and in vivo by a helioxanthin-derivative
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DOI：
10.1016/j.bbrc.2010.04.041
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发表时间：
2010-05-14
影响因子：
3.1
通讯作者：
Chung, Ung-il
中科院分区：
文献类型：
--
作者：
Nakajima, Keiji;Komiyama, Yusuke;Chung, Ung-il
为了利用骨再生医学有效治疗严重的骨缺损，必须开发一种能有效诱导骨形成的小化合物。我们之前报道了4-（4-甲氧基苯基）吡啶[40,30:4,5]噻吩[2,3-b]吡啶-2-羧酰胺（TH）的体外成骨作用，这是一种helioxanthin衍生物。在这里，我们报道了TH在体内和体外的成骨作用。在头骨和跖骨器官培养中th诱导的新骨形成。一种利用MC3T3-E1细胞进行成骨细胞分化的新型监测系统显示，TH从α - tcp骨水泥中释放，并且这种释放持续了一个多月。最后，将含有TH的α - tcp载体植入小鼠颅骨缺损，4周后缺损内新骨面积增加。含有th的支架可能有助于建立更有效的骨再生系统。(C) 2010爱思唯尔公司版权所有。
To effectively treat serious bone defects using bone-regenerative medicine, a small chemical compound that potently induces bone formation must be developed. We previously reported on the osteogenic effect of 4-(4-methoxyphenyl)pyrido[40,30:4,5]thieno[2,3-b]pyridine-2-carboxamide (TH), a helioxanthin-derivative, in vitro. Here, we report on TH's osteogenic effects ex vivo and in vivo. TH-induced new bone formation in both calvarial and metatarsal organ cultures. A novel monitoring system of osteoblastic differentiation using MC3T3-E1 cells revealed that TH was released from alpha-TCP bone cement and this release continued for more than one month. Lastly, the implantation of the alpha-TCP carrier containing TH into defects in mouse skull resulted in increased new bone areas within the defects after 4 weeks. A TH-containing scaffold may help establish a more efficient bone regeneration system. (C) 2010 Elsevier Inc. All rights reserved.