Whole blood screening of antibodies using label-free nanoparticle biophotonic array platform.
Whole blood screening of antibodies using label-free nanoparticle biophotonic array platform.
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DOI：
10.1016/j.bios.2012.04.023
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发表时间：
2012-06
影响因子：
12.6
通讯作者：
R. Olkhov;Robert J. Parker;A. M. Shaw
中科院分区：
文献类型：
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作者：
R. Olkhov;Robert J. Parker;A. M. Shaw
金纳米粒子，局部等离子体阵列生物传感器使用光散射已被用于检测过敏原特异性抗体在全血和血清中。用四种不同的过敏原，猫皮屑（Fel d1），尘螨（Der p1），花生过敏原（Ara h1）和狗皮屑（Can f1）的阵列传感器功能化和免疫动力学测定进行检测各自的抗过敏原IgG抗体。对于Fel d1、Der p1和Ara h1过敏原，观察到对浓度为25 nM的抗体的特异性阳性反应，而Can f1通道作为参考对照。使用第二抗IgG检测抗体进一步增强灵敏度，得到2nM的检测限。结果表明，纳米粒子散射多路复用阵列的潜力，以筛选未准备的血液样品在现场的护理复杂的样品，如全血的测定。
A gold nanoparticle, localised plasmon array biosensor using light scattering has been employed in the detection of allergen-specific antibodies in whole blood and sera. The array sensor was functionalized with four different allergens, cat dander (Fel d1), dust mite (Der p1), peanut allergen (Ara h1) and dog dander (Can f1) and immuno-kinetic assay was performed to detect their respective anti-allergen IgG antibodies. Specific positive responses to antibodies at a concentration of 25nM were observed for Fel d1, Der p1, and Ara h1 allergens, while the Can f1 channel served as a reference control. The sensitivity was further enhanced using a secondary anti-IgG detection antibodies to give a limit of detection of 2nM. The results indicate the potential for nanoparticle scattering multiplexed arrays to screen unprepared blood samples at point-of-care for assays of complex samples such as the whole blood.