Autonomic nerves mediating contractility in the human graafian follicle.
Autonomic nerves mediating contractility in the human graafian follicle.
复制标题
自主神经介导人类格拉夫卵泡的收缩性。
DOI：
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发表时间：
1975
期刊：
影响因子：
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通讯作者：
B. Walles
中科院分区：
文献类型：
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作者：
C. Owman;N. Sjöberg;K. Svensson;B. Walles
荧光组织化学显示，人卵巢广泛地由肾上腺素能神经供应，其中许多与血管无关，并且以暗示局部神经支配的方式与卵泡紧密接触（Owman，Rosengren & Sjl= 0.erg，1967）。自从电子显微镜证实卵泡外膜含有平滑肌细胞以来，这些肾上腺素能神经受到了特别的关注（Okamura，Virutamasen，Wright & Wallach，1972）。为了阐明光滑肌肉组织是否参与Graafian卵泡的收缩，以及神经机制是否可以影响这种功能，我们对例因疼痛、出血、子宫肌瘤或宫颈癌前浸润而行腹式子宫切除术的32例患者进行了研究。将卵泡解剖出来，在冰冷的Krebs-Ringer溶液中运输到实验室（15分钟），在振动切片机中在0 ℃下以30 g=mm的量在没有预先固定的情况下切片，并根据乙醛酸组织荧光方法进一步处理，用于肾上腺素能神经的灵敏可视化（Lindvall & Bjl= 0“）。
klund，1974）。在整个Graafian卵泡壁中发现了绿色荧光的肾上腺素能神经末梢，基本上以单细胞方式运行（PI。1，图1），除了最衰减的顶点区域。远离顶端，非血管神经末梢的数量增加，并在卵泡的卵巢内部分最高。通过电子显微镜在卵泡材料中也证实了肾上腺素能神经，卵泡材料首先在含有100 μ g=mg假肾上腺素能递质5-羟基多巴胺/ml的Krebsp=m-Ringer缓冲液中孵育30分钟，随后在3%KMn 〇 4中固定（Hökfelt，1968）。通过这种处理，肾上腺素能末梢变得选择性标记，并通过存在许多直径约50 nm的致密核心囊泡来区分。这些终末通常以小束的形式运行，部分被雪旺细胞包围，可以在约100 nm内接近平滑肌细胞（PI。2，图3）。偶尔，在距离肌细胞膜仅20 nm处发现裸露轴突静脉曲张（PL 2，图4），表明可能存在功能性神经效应器关系。也发现了少量的神经末梢，仅具有无颗粒的50 nm囊泡，并且还发现这些轴突与平滑肌细胞（PI）紧密并置（20 nm）。2，图5）。这种非肾上腺素能神经可能与光学显微镜观察到的含乙酰胆碱酯酶的纤维相同（PI。1，图2）在组织中
Fluorescence histochemistry has shown that the human ovary is extensively supplied by adrenergic nerves, many of which are not associated with blood vessels and run in close contact with the follicles in a manner suggesting a local innervation (Owman, Rosengren & Sjl=o"erg,1967). Particular attention has been paid to these adrenergic nerves since it became established by electron microscopy that the theca externa of the follicle contains smooth muscle cells (Okamura, Virutamasen, Wright & Wallach, 1972). In order to elucidate whether the smooth musculature is involved in the contractility of the Graafian follicle, and whether neurogenic mechanisms can influence this function, a study was performed on material obtained from 32p=m-48-year-oldpatients undergoing abdominal hysterectomy because of pains, bleeding, uterine myoma, or preinvasive carcinoma of the cervix. The follicles were dissected out, transported to the laboratory in ice-cold Krebs-Ringer solution (15 min), sectioned without previous fixation at 30 g=mm in a Vibratome at 0s=degC and further processed according to the glyoxylic acid histofluorescence method for sensitive visualiza¬ tion of adrenergic nerves (Lindvall & Bjl=o"
klund, 1974). Green-fluorescent adrenergic nerve terminals, running in an essentially meridional fashion, were found in the wall of the whole Graafian follicle (PI. 1, Fig. 1) except for the most attenuated apex region. Away from the apex, the number of non-vascular nerve terminals increased and was highest in the intra-ovarian part of the follicle. Adrenergic nerves were also demonstrated by electron microscopy in follicular material first incubated for 30 min in a Krebsp=m-Ringer buffer solution containing 100 g=mg of the false adrenergic transmitter, 5-hydroxydopamine/ml, and subsequently fixed in 3% KMn04 (Hökfelt, 1968). With this treatment the adrenergic terminals become selectively marked and are distinguished by the presence of numerous dense-cored vesicles, approximately 50 nm in diameter. Such terminals, often running in small bundles partly enclosed by a Schwann cell, were seen to approach smooth muscle cells to within about 100 nm (PI. 2, Fig. 3). Occasionally, naked-axon varicosities were found only 20 nm from the membrane of a muscle cell (PL 2, Fig. 4), indicating the possibility for functional neuro-effector relations. A smaller number of nerve terminals was also found with exclusively agranular 50 nm vesicles, and these axons were also found in close apposition (20 nm) with smooth muscle cells (PI. 2, Fig. 5). Such non-adrenergic nerves are probably identical with the acetylcholinesterasecontaining fibres that were seen by light microscopy (PI. 1, Fig. 2) in tissues