Monoclonal antibody specific for IDH1 R132H mutation
Monoclonal antibody specific for IDH1 R132H mutation
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DOI：
10.1007/s00401-009-0595-z
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发表时间：
2009-11-01
影响因子：
12.7
通讯作者：
von Deimling, Andreas
中科院分区：
文献类型：
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作者：
Capper, David;Zentgraf, Hanswalter;von Deimling, Andreas
IDH1 R132H突变发生在大约70%的星形细胞瘤和少突胶质细胞肿瘤中。我们研制了一种针对IDH1 R132H突变的鼠单抗。在此，我们在免疫印迹和福尔马林固定石蜡包埋肿瘤标本的组织切片上显示了该抗体的高度特异性和敏感性。这种抗体在肿瘤分类、检测单个浸润性肿瘤细胞和表征突变的IDH1蛋白的细胞作用方面非常有用。
IDH1 R132H mutations occur in approximately 70% of astrocytomas and oligodendroglial tumors. We developed a mouse monoclonal antibody targeting the IDH1 R132H mutation. Here, we show the high specificity and sensitivity of this antibody on Western blots and tissue sections from formalin fixed paraffin embedded tumor specimens. This antibody is highly useful for tumor classification, in detecting single infiltrating tumor cells and for the characterization of the cellular role of mutant IDH1 protein.