CD40 ON HUMAN ENDOTHELIAL-CELLS - INDUCIBILITY BY CYTOKINES AND FUNCTIONAL REGULATION OF ADHESION MOLECULE EXPRESSION
CD40 ON HUMAN ENDOTHELIAL-CELLS - INDUCIBILITY BY CYTOKINES AND FUNCTIONAL REGULATION OF ADHESION MOLECULE EXPRESSION
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DOI：
10.1073/pnas.92.10.4342
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发表时间：
1995-05-09
影响因子：
11.1
通讯作者：
POBER, JS
中科院分区：
文献类型：
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作者：
KARMANN, K;HUGHES, CCW;POBER, JS
通过单克隆抗体结合和荧光流式细胞术定量检测，培养的人脐静脉内皮细胞（EC）低水平表达CD40抗原。用最佳浓度的肿瘤坏死因子、白细胞介素1、干扰素β或干扰素γ治疗24小时后，EC上的表达水平增加约3倍；当与肿瘤坏死因子或白细胞介素1联合使用时，两种干扰素对CD40的诱导均大于加性诱导。CD40的表达在细胞因子处理后8小时内增加，并在72小时内继续增加。重组小鼠CD40配体的三聚体形式作用于人EC，增加白细胞粘附分子的表达，包括e -选择素、血管细胞粘附分子1和细胞间粘附分子1。CD40可在正常皮肤微血管EC上进行免疫细胞化学检测。我们得出结论，内皮细胞CD40可能在t细胞介导的炎症反应中发挥信号受体的作用。
Cultured human umbilical vein endothelial cells (EC) constitutively express a low level of CD40 antigen as detected by monoclonal antibody binding and fluorescence flow cytometric quantitation. The level of expression on EC is increased about 3-fold following 24 h treatment with optimal concentrations of tumor necrosis factor, interleukin 1, interferon beta, or interferon gamma; both interferons show greater than additive induction of CD40 when combined with tumor necrosis factor or interleukin 1. Expression of CD40 increases within 8 h of cytokine treatment and continues to increase through 72 h. A trimeric form of recombinant murine CD40 ligand acts on human EC to increase expression of leukocyte adhesion molecules, including E-selectin, vascular cell adhesion molecule 1, and intercellular adhesion molecule 1. CD40 may be detected immunocytochemically on human microvascular EC in normal skin. We conclude that endothelial CD40 may play a role as a signaling receptor in the development of T-cell-mediated inflammatory reactions.