Overexpression of MhYTP2 enhances apple water-use efficiency by activating ABA and ethylene signaling
Overexpression of MhYTP2 enhances apple water-use efficiency by activating ABA and ethylene signaling
复制标题
MhYTP2 的过表达通过激活 ABA 和乙烯信号传导提高苹果水分利用效率
DOI：
10.1016/j.envexpbot.2018.10.018
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发表时间：
2019-01-01
影响因子：
5.7
通讯作者：
Ma, Fengwang
中科院分区：
文献类型：
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作者：
Liu, Changhai;Guo, Tianli;Ma, Fengwang
作物育种的一个关键组成部分是鉴定与提高水分利用效率（WUE）有关的基因。我们的特点MhYTP 2，从平邑甜茶，编码RNA结合蛋白的基因克隆，以研究其在WUE中的作用。在充分浇水和干旱条件下，该基因的过表达增强了转基因植物的WUE，它们比未转化的野生型植物积累了更多的生物量。这可能是由于提高光合速率和根系水分吸收，气孔孔径的增加，导致阿坝水平的升高和激活乙烯信号。MhYTP 2主要在苹果根中表达。我们感兴趣地注意到，在我们的嫁接实验中，当35S：MhYTP 2植物用作砧木而不是用作接穗时，光合速率、瞬时WUE和根水势更高。我们的研究结果表明，MhYTP 2有一个更重要的作用，在根比在地上部分的植物，这意味着该基因可用于提高水分利用效率的苹果砧木，并可能，砧木的其他嫁接适合的作物。
A critical component of crop breeding is the identification of genes involved in improving water-use efficiency (WUE). We characterized MhYTP2, a gene cloned from Malus hupehensis that encodes an RNA-binding protein, to examine its role in WUE. Under both well-watered and drought conditions, overexpression of this gene enhanced WUE in transgenic plants and they accumulated more biomass than untransformed wild-type plants. This increase in WUE was probably due to enhancement of photosynthetic rate and root water uptake, and a reduction in stomatal aperture that resulted from elevated level of ABA and activated ethylene signaling. MhYTP2 was predominantly expressed in apple roots. We noted with interest that the rate of photosynthesis, instantaneous WUE, and root water potential were higher when 35S:MhYTP2 plants were used as rootstocks rather than as scions in our grafting experiments. Our results indicated that MhYTP2 has a more crucial role in the roots than in the aerial parts of the plant, and it implies that this gene can be used for improving WUE by apple rootstocks and, potentially, rootstocks of other graft-suitable crops.