Establishment and characterization of a spontaneously immortalized mouse ameloblast-lineage cell line
Establishment and characterization of a spontaneously immortalized mouse ameloblast-lineage cell line
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DOI：
10.1016/s0006-291x(03)01467-0
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发表时间：
2003-09-05
影响因子：
3.1
通讯作者：
Sugiyama, T
中科院分区：
文献类型：
--
作者：
Nakata, A;Kameda, T;Sugiyama, T
牙齿的发育受上皮成釉细胞和间充质成牙本质细胞的共同调控。成釉细胞分泌釉质基质，对釉质形成至关重要。虽然有一些关于通过导入病毒癌基因建立永生化成釉细胞样细胞的报道，但我们试图建立一种自发永生化成釉细胞系，保持细胞类型特异性，包括诱导体外生物矿化的能力。已建立的细胞系（成釉细胞系细胞; ALC）在长期培养中保持了几种成釉细胞特异性基因（釉原蛋白、Tuftelin和釉蛋白）的表达。他们形成钙化结节诱导后，由培养基切换SMEM DMEM，具有高水平的碱性磷酸酶活性。TGF-β处理增强了钙化结节形成的大小和数量。将ALC植入裸鼠皮下6周后，异位观察到釉质上皮样结构形成、软骨形成和钙化。这些数据表明，ALC是一个有用的实验工具，分析成釉细胞的性质。(C)2003年爱思唯尔公司All rights reserved.
Tooth development was cooperatively regulated by the epithelial ameloblasts and mesenchymal odontoblasts. Ameloblasts secrete enamel matrix, critical for enamel formation. While there are several reports about establishment of immortalized ameloblast-like cells by introducing viral oncogene, we tried to establish a spontaneously immortalized ameloblast-lineage cell line, maintaining the cell type specific character, including the ability to induce in vitro bio-mineralization. The established cell line (ameloblast-lineage cell; ALC) maintained the expression of several ameloblast specific genes (Amelogenin, Tuftelin, and Enamelin) in long-term culture. They formed calcified nodules after the induction by medium switching from SMEM to DMEM, having high-level alkaline-phosphatase activity. The size and number of calcified nodule formation were enhanced by TGF-beta treatment. Six weeks after sub-cutaneous implantation of ALC to athymic nude mice, we ectopically observed enamel epithelium like structure formation, chondrogenesis, and calcification. These data indicate that ALC is a useful experimental tool to analyze ameloblast character. (C) 2003 Elsevier Inc. All rights reserved.