Safeguarding Nonhuman Primate iPS Cells With Suicide Genes
Safeguarding Nonhuman Primate iPS Cells With Suicide Genes
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DOI：
10.1038/mt.2011.51
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发表时间：
2011-09-01
影响因子：
12.4
通讯作者：
Kiem, Hans-Peter
中科院分区：
文献类型：
--
作者：
Zhong, Bonan;Watts, Korashon L.;Kiem, Hans-Peter
由于再生医学的巨大潜力，产生诱导多能干细胞（iPS）的技术的发展构成了最令人兴奋的科学突破之一。然而，iPS细胞相关产品的安全性是临床转化的主要问题。插入诱变、iPS细胞或其衍生物的可能致癌转化、或分化的iPS细胞与未分化细胞的污染导致畸胎瘤的形成，仍然存在相当大的障碍。在这里，我们证明了自杀基因的效用，以保障iPS细胞及其衍生物。我们发现自杀基因可以在体外和体内控制iPS细胞的细胞命运，而不干扰其多能性和自我更新能力。本研究将有助于在临床高度相关的大型动物模型中评估iPS细胞技术的安全性，并进一步有益于人iPS细胞的临床应用。
The development of technology to generate induced pluripotent stem (iPS) cells constitutes one of the most exciting scientific breakthroughs because of the enormous potential for regenerative medicine. However, the safety of iPS cell-related products is a major concern for clinical translation. Insertional mutagenesis, possible oncogenic transformation of iPS cells or their derivatives, or the contamination of differentiated iPS cells with undifferentiated cells, resulting in the formation of teratomas, have remained considerable obstacles. Here, we demonstrate the utility of suicide genes to safeguard iPS cells and their derivatives. We found suicide genes can control the cell fate of iPS cells in vitro and in vivo without interfering with their pluripotency and self-renewal capacity. This study will be useful to evaluate the safety of iPS cell technology in a clinically highly relevant, large animal model and further benefit the clinical use of human iPS cells.