The effects of cross-circulation and charcoal hemoperfusion on adenylate energy charge of the remnant liver after major hepatic resection.
The effects of cross-circulation and charcoal hemoperfusion on adenylate energy charge of the remnant liver after major hepatic resection.
复制标题
交叉循环和木炭血液灌流对主要肝切除术后残肝腺苷酸能量的影响。
作者：
M. Ukikusa;K. Ozawa;Y. Shimahara;K. Sawanishi;T. Tobe
研究了肝支持对兔肝大切除后残肝腺苷酸能量电荷(ATP + 1/2 ADP) / (ATP + ADP + AMP)的影响。本研究强调采用同种或异体肝脏预防肝大切除后残肝能量电荷下降的原则。能量电荷水平为细胞提供了一种非常敏感的细胞内控制机制。来自生物合成序列的调节酶在低电荷水平下表现出很少的活性，而在高电荷值下它们的活性急剧增加。70%肝切除后24小时，残肝能量电荷水平由正常水平0.843下降到0.767，最大程度下降。24小时切除70%肝的家兔与正常家兔的全身血液交叉循环1小时后能量电荷水平由0.767提高到0.828。炭血灌流1小时后，血药浓度由0.767上升至0.801。尽管两种肝脏支持方式都有助于恢复能量电荷水平，但上述结果表明，在残肝再生过程中，交叉循环可能比木炭血液灌流更有效地促进生物合成。
The effect of liver support on the adenylate energy charge (ATP + 1/2 ADP) / (ATP + ADP + AMP) of the remnant liver after major hepatic resection was studied in rabbits. The present study emphasizes the principle of the use of homologous or heterologous liver for preventing the decrease in the energy charge of the remnant liver after major hepatic resection. The energy charge level provides the cell with a very sensitive intracellular control mechanism. Regulatory enzymes from biosynthetic sequences exhibit very little activity at low levels of energy charge, and their activities increase sharply at high charge values. The energy charge level of the remnant liver maximally decreased from the normal level of 0.843 to 0.767 at 24 hours after 70% hepatectomy. The energy charge level increased from 0.767 to 0.828 after one hour of cross-circulation of systemic blood between the 24-hour, 70% hepatectomized rabbit and a normal rabbit. An increase from 0.767 to 0.801 also occurred after one hour of charcoal hemoperfusion. Although either means of liver support serves to restore energy charge levels, the above results suggest that cross-circulation may be more effective than charcoal hemoperfusion for biosynthesis in the regenerative processes of the remnant liver.