Long- and short-term intravital imaging reveals differential spatiotemporal recruitment and function of myelomonocytic cells after spinal cord injury
Long- and short-term intravital imaging reveals differential spatiotemporal recruitment and function of myelomonocytic cells after spinal cord injury
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DOI：
10.1113/jphysiol.2013.256388
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发表时间：
2013-10-01
影响因子：
5.5
通讯作者：
Debarbieux, Franck
中科院分区：
文献类型：
--
作者：
Fenrich, Keith K.;Weber, Pascal;Debarbieux, Franck
脊髓损伤(SCI)后，常驻细胞和外周血粒单核细胞被招募到损伤部位，并在损伤进展中发挥作用。这些细胞对清除细胞碎片很重要，并能在体外调节轴突的回缩和生长。然而，它们准确的时空招募动态尚不清楚，它们在脊髓损伤后各自扮演的角色仍然存在激烈的争论。通过对成年脊髓损伤小鼠的慢性定量活体双光子显微镜观察，我们发现浸润性溶菌酶M(LysM(+))和驻留的CD11c(+)粒单核细胞具有不同的时空募集特征，并随着时间的推移表现出形态、运动性、吞噬活性和轴突相互作用模式的变化。这项研究首次在体内描述了炎性和滞留的单核细胞进入损伤的脊髓以及它们与切断的轴突的相互作用，并强调了精确的时间选择和特定细胞群的靶向在开发脊髓损伤治疗方法中的重要性。
After spinal cord injury (SCI), resident and peripheral myelomonocytic cells are recruited to the injury site and play a role in injury progression. These cells are important for clearing cellular debris, and can modulate the retraction and growth of axons in vitro. However, their precise spatiotemporal recruitment dynamics is unknown, and their respective roles after SCI remain heavily debated. Using chronic, quantitative intravital two-photon microscopy of adult mice with SCI, here we show that infiltrating lysozyme M (LysM(+)) and resident CD11c(+) myelomonocytic cells have distinct spatiotemporal recruitment profiles, and exhibit changes in morphology, motility, phagocytic activity and axon interaction patterns over time. This study provides the first in vivo description of the influx of inflammatory and resident myelomonocytic cells into the injured spinal cord and their interactions with cut axons, and underscores the importance of precise timing and targeting of specific cell populations in developing therapies for SCI.