Apoptosis of bovine neutrophils during mastitis experimentally induced with Escherichia coli or endotoxin.
Apoptosis of bovine neutrophils during mastitis experimentally induced with Escherichia coli or endotoxin.
复制标题
用大肠杆菌或内毒素实验诱导乳腺炎期间牛中性粒细胞的凋亡。
DOI：
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发表时间：
2002
影响因子：
1
通讯作者：
C. Burvenich
中科院分区：
文献类型：
--
作者：
K. Van Oostveldt;G. Tomita;M. Paape;A. Capuco;C. Burvenich
目的
确定在使用大肠杆菌或大肠杆菌内毒素实验诱导的乳房炎期间，中性粒细胞的凋亡是否加速，以及对大肠杆菌或内毒素的反应是否存在明显差异。
动物
11头健康泌乳荷斯坦奶牛。
程序
在乳房内接种大肠杆菌或内毒素后的不同时间间隔从奶牛中采集血样。测定体外孵育3小时后检测到的凋亡中性粒细胞的百分比。异硫氰酸黄绿素标记的annexin-V结合碘化丙啶用于区分中性粒细胞的凋亡和坏死。在采集血样时测定总循环白细胞计数和分类计数以及直肠温度。在挤奶时测定产奶量和乳汁体细胞计数。
结果
接种内毒素并不加速体外诱导中性粒细胞凋亡。然而，大肠杆菌的接种增加了凋亡的中性粒细胞的百分比。接种后18小时，20%的中性粒细胞凋亡，而接种前为5%。接种大肠杆菌或内毒素后，乳汁体细胞数和直肠温度升高，产奶量和白细胞总数下降，未成熟中性粒细胞百分比升高。然而，内毒素诱导的乳腺炎的反应动力学更快，更严重，持续时间更短。
结论和临床相关性
在体外诱导的中性粒细胞凋亡加速，只有在大肠杆菌诱导的乳腺炎，而不是在内毒素诱导的乳腺炎。内毒素接种作为研究奶牛大肠杆菌性乳房炎的模型应谨慎对待。
OBJECTIVE
To determine whether apoptosis of neutrophils was accelerated during mastits experimentally induced by use of Escherichia coli or E coli endotoxin and whether differences were apparent in the response to E coli or endotoxin.
ANIMALS
11 healthy lactating Holstein cows.
PROCEDURE
Blood samples were collected from cows at various intervals after intramammary inoculation with E coli or endotoxin. Percentage of apoptotic neutrophils detected after in vitro incubation for 3 hours was determined. Fluorescein isothiocyanate-labeled annexin-V in combination with propidium iodide was used to distinguish apoptosis and necrosis of neutrophils. Total and differential circulating leukocyte counts and rectal temperature were determined at the time of collection of blood samples. Milk yield and milk somatic cell counts were determined at the time of milking.
RESULTS
Inoculation of endotoxin did not accelerate in vitro induction of neutrophil apoptosis. However, inoculation of E coli increased the percentage of apoptotic neutrophils. At 18 hours after inoculation, 20% of the neutrophils were apoptotic, compared with 5% before inoculation. Milk somatic cell count and rectal temperature increased, milk production and total leukocyte count decreased, and percentage of immature neutrophils increased after inoculation with E coli or endotoxin. However, kinetics of the responses were more rapid, more severe, and of shorter duration during endotoxin-induced mastitis.
CONCLUSIONS AND CLINICAL RELEVANCE
In vitro induction of apoptosis of neutrophils was accelerated only during E coli-induced mastitis and not during endotoxin-induced mastitis. Endotoxin inoculation as a model for studying coliform mastitis in dairy cows should be viewed with caution.
影响因子：
15.9
作者：
SAVILL, JS;WYLLIE, AH;HASLETT, C
通讯作者：
HASLETT, C