Tanshinone IIA exhibits anti-inflammatory and antioxidative effects in LPS-stimulated bovine endometrial epithelial cells by activating the Nrf2 signaling pathway
Tanshinone IIA exhibits anti-inflammatory and antioxidative effects in LPS-stimulated bovine endometrial epithelial cells by activating the Nrf2 signaling pathway
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DOI：
10.1016/j.rvsc.2021.03.004
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发表时间：
2021-03-06
影响因子：
2.4
通讯作者：
Cao, Rongfeng
中科院分区：
文献类型：
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作者：
Fu, Kaiqiang;Feng, Chao;Cao, Rongfeng
子宫内膜炎是产牛的一种主要疾病，也可在分娩后由条件致病菌引起。牛子宫内膜上皮细胞的完整性和活性决定了子宫内膜炎的发生。丹参酮Ⅱ A是从丹参中提取的一种化合物，具有抗炎作用。本研究的目的是探讨丹参酮IIA在LPS诱导的bEECs炎症反应中的抗炎作用。结果表明，丹参酮IIA可抑制LPS诱导的考克斯-2和iNOS的mRNA表达，降低IL-18、TNF-α、IL-6和IL-8的表达。丹参酮IIA可抑制MDA的生成，提高CAT和SOD的活性。为了评价丹参酮Ⅱ A的抗炎机制，我们检测了Nrf 2的激活。结果表明，丹参酮IIA可显著激活Nrf 2信号通路。综上所述，丹参酮IIA通过激活Nrf 2信号通路发挥抗炎和抗氧化作用。
Endometritis is a major disease in productive bovines, and is also caused by conditional pathogens after delivery. The integrity and activity of bovine endometrial epithelial cells (bEECs) determine the development of endometritis. Tanshinone IIA, a compound purified from Salvia miltiorrhiza bunge, has been reported to have antiinflammatory effects. The aim of this study was to investigate the anti-inflammatory effects of tanshinone IIA in the LPS-induced inflammatory response of bEECs. The results showed that tanshinone IIA inhibited the mRNA expression levels of COX-2 and iNOS, and reduced the expression levels of IL-18, TNF-alpha, IL-6 and IL-8 induced by LPS. In addition, we found that tanshinone IIA inhibited the level of MDA, but increased the activities of CAT and SOD. To evaluate the anti-inflammatory mechanism of tanshinone IIA, we examined the activation of Nrf2. The results showed that the Nrf2 signaling pathway was significantly activated by tanshinone IIA. In conclusion, these results showed that tanshinone IIA exhibited anti-inflammatory and antioxidative effects by activating the Nrf2 signaling pathway.