Singlet Oxygen Sensor Green®: Photochemical Behavior in Solution and in a Mammalian Cell
Singlet Oxygen Sensor Green®: Photochemical Behavior in Solution and in a Mammalian Cell
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DOI：
10.1111/j.1751-1097.2011.00900.x
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发表时间：
2011-05-01
影响因子：
3.3
通讯作者：
Ogilby, Peter R.
中科院分区：
文献类型：
--
作者：
Gollmer, Anita;Arnbjerg, Jacob;Ogilby, Peter R.
活性氧，特别是单线态氧的高效、高选择性荧光探针的研制具有重要意义。在这项研究中，单线态氧传感器绿色（SOSG），一种市售的单线态氧荧光探针的光化学行为进行了检查。尽管发表了相反的声明，但本文提供的数据表明，SOSG实际上可以掺入活的哺乳动物细胞中。但是，由于多种原因，在使用SOSG时必须谨慎。首先，它表明，SOSG和单线态氧之间的反应的直接产物是，本身，一个有效的单线态氧光敏剂。其次，SOSG似乎有效地结合蛋白质，这反过来又可以影响细胞的摄取以及细胞中的行为。因此，SOSG的不正确使用可能会产生关于光敏单线态氧产生产率的误导性数据，并且还可能导致正在研究的系统中的光氧合依赖性不良反应。
The development of efficient and selective luminescent probes for reactive oxygen species, particularly for singlet molecular oxygen, is currently of great importance. In this study, the photochemical behavior of Singlet Oxygen Sensor Green (R) (SOSG), a commercially available fluorescent probe for singlet oxygen, was examined. Despite published claims to the contrary, the data presented herein indicate that SOSG can, in fact, be incorporated into a living mammalian cell. However, for a number of reasons, caution must be exercised when using SOSG. First, it is shown that the immediate product of the reaction between SOSG and singlet oxygen is, itself, an efficient singlet oxygen photosensitizer. Second, SOSG appears to efficiently bind to proteins which, in turn, can influence uptake by a cell as well as behavior in the cell. As such, incorrect use of SOSG can yield misleading data on yields of photosensitized singlet oxygen production, and can also lead to photooxygenation-dependent adverse effects in the system being investigated.