Death and Survival Signals in Photodynamic Therapy
Death and Survival Signals in Photodynamic Therapy
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DOI：
10.1007/978-1-60761-697-9_2
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发表时间：
2010-01-01
期刊：
影响因子：
--
通讯作者：
Agostinis, Patrizia
中科院分区：
文献类型：
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作者：
Dewaele, Michael;Verfaillie, Tom;Agostinis, Patrizia
光动力疗法（PDT）是一种抗癌方式，其利用通过可见光照射癌组织中积累的光敏染料产生单线态氧和其他活性氧物质。当癌细胞暴露于光动力应激时，多个信号级联被同时激活，并且取决于所产生的ROS的亚细胞位置和氧化损伤的强度，它们决定细胞是否会科普应激并存活或屈服并死亡。已经开发了不同的方法，以允许在形态学，超微结构和生化水平上的细胞死亡子程序的歧视，并仔细检查响应PDT的信号级联。在这里，我们描述了一个有用的技术来表征细胞凋亡和自噬，并监测激活状态的MAPK和Akt-mTOR途径PDT后。
Photodynamic therapy (PDT) is an anticancer modality utilizing the generation of singlet oxygen and other reactive oxygen species through visible light irradiation of a photosensitive dye accumulated in the cancerous tissue. Upon exposure of cancer cells to the photodynamic stress, multiple signaling cascades are concomitantly activated and depending on the subcellular location of the generated ROS and the intensity of the oxidative damage, they dictate whether cells will cope with the stress and survive or succumb and die. Different methodologies have been developed to allow the discrimination of cell death subroutines at the morphological, ultrastructural, and biochemical levels and to scrutinize signaling cascades in response to PDT. Here we describe a selection of useful techniques to characterize apoptosis and autophagy and to monitor the activation status of the MAPK- and Akt-mTOR pathways after PDT.