Pathophysiological function of adrenomedullin and proadrenomedullin N-terminal peptides in adrenal chromaffin cells.
Pathophysiological function of adrenomedullin and proadrenomedullin N-terminal peptides in adrenal chromaffin cells.
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DOI：
10.1291/hypres.26.s71
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发表时间：
2003-02
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影响因子：
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通讯作者：
Hideyuki Kobayashi;T. Yanagita;H. Yokoo;A. Wada
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作者：
Hideyuki Kobayashi;T. Yanagita;H. Yokoo;A. Wada
肾上腺髓质素（AM）和肾上腺髓质素前体N-末端20肽（PAMP 20）家族的肽是在肾上腺髓质中大量表达的多功能肽。这些肽在刺激肾上腺嗜铬细胞时通过受调节的胞吐沿着儿茶酚胺释放。它们也在培养过程中逐渐释放，并且这种释放由3 '，5'-环腺苷酸（cAMP）依赖性途径刺激。嗜铬细胞缺氧时AM的表达和释放增加。神经生长因子诱导嗜铬细胞瘤PC 12细胞向神经元分化过程中AM的表达减少。另一方面，PAMP 20和PAMP 12通过干扰烟碱胆碱能受体来抑制儿茶酚胺的释放和合成。AM增加肾上腺中的血流量，并引起儿茶酚胺的逐渐释放，但在细胞刺激时不改变调节的胞吐作用。目前的数据表明，这些肽的表达受到细胞内信号通路的调节，并在各种生理和病理条件下发生变化。AM和PAMP 20家族多肽具有不同的生理功能。PAMP 20和PAMP 12是以自分泌方式调节嗜铬细胞功能的内源性肽，而AM可能主要以旁分泌方式调节血管细胞功能。
Adrenomedullin (AM) and peptides of the proadrenomedullin N-terminal 20 peptide (PAMP20) family are multifunctional peptides abundantly expressed in the adrenal medulla. These peptides are released by regulated exocytosis along with catecholamines upon stimulation of adrenal chromaffin cells. They are also released gradually during culture, and this release is stimulated by a 3',5'-cyclic adenosine monophosphate (cAMP)-dependent pathway. The expression and release of AM increase under hypoxia in chromaffin cells. The expression of AM in pheochromocytoma PC12 cells is reduced during neuronal differentiation with nerve growth factor. On the other hand, PAMP20 and PAMP12 suppress catecholamine release and synthesis by interfering with nicotinic cholinergic receptors. AM increases blood flow in the adrenal gland, and causes a gradual release of catecholamine, but does not modify regulated exocytosis upon the stimulation of cells. Current data indicate that the expression of these peptides is regulated by intracellular signaling pathways, and changes under various physiological and pathological conditions. AM and PAMP20 family peptides have distinct physiological functions. PAMP20 and PAMP12 are endogenous peptides that modulate chromaffin cell function in an autocrine manner, whereas AM may mainly regulate vascular cell function in a paracrine manner.