IMMORTALIZATION OF MURINE MICROGLIAL CELLS BY A V-RAF/V-MYC CARRYING RETROVIRUS
IMMORTALIZATION OF MURINE MICROGLIAL CELLS BY A V-RAF/V-MYC CARRYING RETROVIRUS
复制标题
DOI：
10.1016/0165-5728(90)90073-v
复制
发表时间：
1990-05-01
影响因子：
3.3
通讯作者：
BISTONI, F
中科院分区：
文献类型：
--
作者：
BLASI, E;BARLUZZI, R;BISTONI, F
通过用携带v-raf/v-myc癌基因的逆转录病毒（J2）感染原代小胶质细胞培养物，产生了鼠细胞系（BV-2）。BV-2细胞表达非特异性酯酶活性，吞噬能力和缺乏过氧化物酶活性。这些细胞分泌溶菌酶，并在适当刺激后，还分泌白细胞介素1和肿瘤坏死因子。此外，BV-2细胞在用干扰素-γ处理时表现出自发的抗念珠菌活性和获得的杀肿瘤活性。BV-2细胞的MAC 1和MAC 2抗原呈阳性，而MAC 3、胶质细胞酸性蛋白（GFAP）和半乳糖苷（GC）抗原呈阴性。由于BV-2细胞保留了新鲜分离的小胶质细胞的大部分形态，表型和功能特性，我们可以得出结论，J2病毒感染导致了活性小胶质细胞的永生化。
A murine celline (BV-2) has been generated by infecting primary microglial cell cultures with a v-raf/v-myc oncogene carrying retrovirus (J2). BV-2 cells expressed nonspecific esterase activity, phagocytic ability and lacked peroxidase activity. Such cells secreted lysozyme and, following appropriate stimulation, also interleukin 1 and tumor necrosis factor. Furthermore, BV-2 cells exhibited spontaneous anti-Candida activity and acquired tumoricidal activity upon treatment with interferon-.gamma.. Phenotypically, BV-2 cells resulted positive for MAC1 and MAC2 antigens, and negative for MAC3, glial fibrillary acidic protein (GFAP) and galactocerebroside (GC) antigens. Since BV-2 cells retain most of the morphological, phenotypical and functional properties described for freshly isolated microglial cells, we can conclude that J2 virus infection has resulted in the immortalization of active microglial cells.