Contribution of Antibody-based Protein Profiling to the Human Chromosome-centric Proteome Project (C-HPP)
Contribution of Antibody-based Protein Profiling to the Human Chromosome-centric Proteome Project (C-HPP)
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DOI：
10.1021/pr300924j
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发表时间：
2013-06-01
影响因子：
4.4
通讯作者：
Uhlen, Mathias
中科院分区：
文献类型：
--
作者：
Fagerberg, Linn;Oksvold, Per;Uhlen, Mathias
一个以基因为中心的人类蛋白质组计划已经被提出，以以染色体为中心描述人类蛋白质编码基因的特征，以了解人类生物学和疾病。在这里，我们报告了从基因组序列预测的所有基因的蛋白质证据，基于文献中的手动注释(UniProt)，细胞、组织和器官中基于抗体的图谱，以及使用不同来源的人类细胞系的下一代测序分析转录图谱。我们估计，69%(n=13985)的人类蛋白质编码基因有很好的证据证明蛋白质的存在，其中23%只在核糖核酸水平上有证据，7%仍然缺乏实验证据。对表达模式的分析表明，很少有蛋白质证据以染色体为中心的方式被可视化，作为新版人类蛋白质图谱的一部分(www.Proteatlas.org)。
A gene-centric Human Proteome Project has been proposed to characterize the human protein-coding genes in a chromosome-centered manner to understand human biology and disease. Here, we report on the protein evidence for all genes predicted from the genome sequence based on manual annotation from literature (UniProt), antibody-based profiling in cells, tissues and organs and analysis of the transcript profiles using next generation sequencing in human cell lines of different origins. We estimate that there is good evidence for protein existence for 69% (n = 13985) of the human protein-coding genes, while 23% have only evidence on the RNA level and 7% still lack experimental evidence. Analysis of the expression patterns shows few regards to protein evidence is visualized in a chromosome-centric manner as part of a new version of the Human Protein Atlas (www.proteinatlas.org).