INSITU IMMUNOFLUORESCENT LABELING OF RIBULOSE-1, 5-BISPHOSPHATECARBOXYLASE IN LEAVES OF C3 AND C4 PLANTS
INSITU IMMUNOFLUORESCENT LABELING OF RIBULOSE-1, 5-BISPHOSPHATECARBOXYLASE IN LEAVES OF C3 AND C4 PLANTS
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DOI：
10.1071/pp9770523
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发表时间：
1977-01-01
期刊：
影响因子：
--
通讯作者：
OSMOND, CB
中科院分区：
文献类型：
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作者：
HATTERSLEY, PW;WATSON, L;OSMOND, CB
使用针对小麦和菠菜核酮糖-1,5-二磷酸羧化酶 (RuP2Case) 的抗体，通过免疫荧光（使用间接技术）在 40 个 C3 和 C4 物种以及 1 个景天酸代谢 (CAM) 植物的手切叶片横切面中定位该酶。样本包括来自 7 个植物科的物种，包括单子叶植物和双子叶植物。在 C3 和 CAM 物种中，当用抗 RuP2Case 标记时，特异性荧光与所有叶绿细胞细胞的叶绿体相关，而在具有经典 C4 叶解剖结构的物种中，RuP2Case 几乎完全位于束鞘 [Kranz 或光合碳还原 (PCR)] 细胞叶绿体中。十种 C4 物种表现出各种类型的非经典 C4 叶解剖结构（Alloteropsis、Aristida、Arundinella、Cyperus、Fimbristylis、Triodia 和 Salsola 类型），并且对于除其中一种类型之外的所有类型，RuP2Case 的免疫荧光标记提供了光合 C 代谢的细胞区室划分和 PCR 区室位置的第一个直接实验证据。 2 个滨藜 C3/C4 杂交个体和黍（假定的 C3/C4 中间体）的叶子表现出 C3 抗体标记反应。
Antibodies raised to wheat and spinach ribulose-1,5-bisphosphate carboxylase (RuP2Case) were used to locate the enzyme in hand-cut leaf blade transections of 40 C3 and C4 spp., and 1 crassulacean acid metabolism (CAM) plant by immunofluorescence (using the indirect technique). The sample includes species from 7 plant families, both monocotyledons and dicotyledons. In C3 and CAM species, specific fluorescence is associated with chloroplasts of all leaf chlorenchymatous cells when labeled with anti-RuP2Case, while in species with classical C4 leaf anatomy RuP2Case is located almost exclusively in bundle sheath [Kranz or photosynthetic carbon reduction (PCR)] cell chloroplasts. Ten C4 species exhibit various types of non-classical C4 leaf anatomy (Alloteropsis, Aristida, Arundinella, Cyperus, Fimbristylis, Triodia and Salsola types) and, for all but 1 of these types, immunofluorescent labeling of RuP2Case provides the first direct experimental evidence of a cellular compartmentation of photosynthetic C metabolism and of the location of PCR compartments. Leaves of 2 Atriplex C3/C4 hybrid individuals and of Panicum milioides, a putative C3/C4 intermediate, exhibited a C3 antibody labeling response.