Induction of apoptosis in HepG2 cells by solanine and Bcl-2 protein
Induction of apoptosis in HepG2 cells by solanine and Bcl-2 protein
复制标题
DOI：
10.1016/j.jep.2007.09.023
复制
发表时间：
2008-01-17
影响因子：
5.4
通讯作者：
Zou, X.
中科院分区：
文献类型：
--
作者：
Ji, Y. B.;Gao, S. Y.;Zou, X.
龙葵（Solanum nigrum Linn.）在中国传统医学中被广泛用于治疗消化系统癌症。龙葵碱是一种从龙葵中分离出来的类固醇生物碱，其抗肿瘤活性已被证实。观察龙葵碱的抗肿瘤作用及其作用机制。采用MTT法测定3种消化系统肿瘤细胞系的IC50。用激光共聚焦显微镜观察其对形貌的影响；流式细胞术（FCM）检测细胞凋亡率和细胞周期；Western blot检测Bcl-2蛋白的表达。结果表明，HepG(2)、SGC-7901和LS-174的IC50分别为14.47、bb50和bb50 μ g/ml；阴性对照细胞形态正常；治疗组出现典型的细胞凋亡迹象。龙葵碱诱导HepG(2)细胞凋亡率分别为6.0、14.4%、17.3%、18.9%和32.2%。细胞周期观察显示，各处理组G(2)/M期细胞消失，S期细胞数量明显增加。Western blot结果显示，茄碱可降低Bcl-2蛋白的表达。因此，龙葵碱诱导HepG2细胞凋亡的靶点可能是通过抑制Bcl-2蛋白的表达介导的。2007爱思唯尔爱尔兰有限公司版权所有。
The nightshade (Solanum nigrum Linn.) has been widely used in Chinese traditional medicine as a remedy for the treatment of digestive system cancer. The anti-tumor activity of solanine, a steroid alkaloid isolated from the nightshade has been demonstrated. To observe the effect of antitumor and mechanism of solanine. The MTT assay was used to evaluate the IC50 on the three digestive system tumor cell lines. The effect on the morphology was observed with a laser confocal microscopy; the rate of apoptosis and the cell cycle were measured using flow cytometry (FCM); the expression of Bcl-2 protein was measured by Western blot. The results show that the IC50 for HepG(2), SGC-7901, and LS-174 were 14.47, >50, and >50 mu g/ml, respectively; the morphology of cells in the negative control was normal; for the treated groups, typical signs for apoptosis were found. The rate of apoptosis in HepG(2) cells induced by solanine was found to be 6.0, 14.4, 17.3, 18.9, and 32.2%, respectively. Observation of the cell cycle showed that cells in the G(2)/M phases disappeared while the number of cells in the S phase increased significantly for treated groups. Western blot showed that solanine decreased the expression of Bcl-2 protein. Therefore, the target of solanine in inducing apoptosis in HepG2 cells seems to be mediated by the inhibition in the expression of Bcl-2 protein. (C) 2007 Elsevier Ireland Ltd. All rights reserved.