Preparation and characterization of keratin-chitosan composite film.
Preparation and characterization of keratin-chitosan composite film.
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DOI：
10.1016/s0142-9612(01)00187-9
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发表时间：
2002-02
期刊：
影响因子：
14
通讯作者：
T. Tanabe;Naoya Okitsu;A. Tachibana;K. Yamauchi
中科院分区：
文献类型：
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作者：
T. Tanabe;Naoya Okitsu;A. Tachibana;K. Yamauchi
将两种生物聚合物的混合溶液浇注在 75% 乙酸中制备角蛋白-壳聚糖复合膜。虽然没有任何添加剂的角蛋白膜非常脆弱，但添加10-30wt%的壳聚糖可产生坚固且柔韧的膜（极限强度：27-34MPa，极限伸长率：4-9%）。甘油（20wt%）还赋予角蛋白膜柔韧性（极限强度：1MPa，极限伸长率：28%）。在含甘油的角蛋白膜中进一步添加壳聚糖可将极限强度提高至 9-14MPa，但对极限伸长率影响不大。这些数据表明，通过适当添加壳聚糖和甘油可以调节角蛋白膜的机械性能。与角蛋白和壳聚糖膜相比，复合膜的防水特性（例如溶胀行为和溶胀后的机械性能）分别得到很大改善。此外，当用膜处理细菌悬浮液时，角蛋白-壳聚糖复合膜以及壳聚糖膜会减少细菌数量，因为细菌不可逆地吸附在膜上。复合膜以及角蛋白和壳聚糖膜支持成纤维细胞附着和增殖，证明是哺乳动物细胞培养的良好基质。
Keratin–chitosan composite film was prepared by casting the mixed solution of both biopolymers in 75% acetic acid. Although keratin film without any additive is very fragile, 10–30wt% of chitosan addition gave strong and flexible film (ultimate strength: 27–34MPa, ultimate elongation: 4–9%). Glycerol (20wt%) also afforded flexibility to keratin film (ultimate strength: 1MPa, ultimate elongation: 28%). Further addition of chitosan to glycerol-containing keratin film increased the ultimate strength to 9–14MPa but gave little effect on ultimate elongation. These data suggest that mechanical properties of keratin film are adjustable by appropriately adding chitosan and glycerol. Waterproof characteristics such as swelling behavior and mechanical properties after swelling were much ameliorated for the composite film compared with keratin and chitosan films, respectively. Furthermore, keratin–chitosan composite film as well as chitosan film decreased bacteria number when the bacteria suspension was treated with a film owing to the irreversible adsorption of bacteria onto the film. The composite film as well as keratin and chitosan films supported fibroblast attachment and proliferation, demonstrating to be a good substrate for mammalian cell culture.