Metagenomic analysis of the canine oral cavity as revealed by high-throughput pyrosequencing of the 16S rRNA gene
Metagenomic analysis of the canine oral cavity as revealed by high-throughput pyrosequencing of the 16S rRNA gene
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DOI：
10.1016/j.vetmic.2012.11.018
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发表时间：
2013-03-23
影响因子：
3.3
通讯作者：
Weese, J. Scott
中科院分区：
文献类型：
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作者：
Sturgeon, Amy;Stull, Jason W.;Weese, J. Scott
犬口腔微生物组的有效表征对于理解口腔细菌在健康中的作用以及提供对牙周病早期诊断和治疗策略的见解至关重要。到目前为止，表征一直局限于基于克隆的测序和传统的文化为基础的研究，这通常低估了社区的多样性，由于其固有的偏见，在他们的方法。焦磷酸测序是一种不依赖克隆和培养的测序方法，它消除了分析中的这些偏倚因素，并能够对微生物种群进行广泛的测序。在这份报告中，焦磷酸测序的16S rRNA基因被用来检查口腔样本，从6个健康的狗，努力确定社区成员，多样性和人畜共患病的影响。焦磷酸测序揭示了平均（SD）226（59）个操作分类单位（OTU，97%相似性），代表来自13个细菌门的181个属。拟杆菌门（60.2%）、变形菌门（20.8%）、厚壁菌门（11.4%）、梭菌门（4.7%）和螺旋体门（1.7%）占优势。最常见的属是卟啉单胞菌（39.2%的序列），梭杆菌（4.5%），二氧化碳噬纤维菌（3.8%），德克夏（3.7%），莫拉菌（3.3%），和Bergeyella（2.7%）。在所有样品中存在对应于38个独特属级鉴定的五十六个OTU，这支持了健康狗之间存在稳定的核心微生物组的概念。在所有犬中均检测到潜在的人畜共患病和牙周细菌，并强调了口腔微生物菌群的人畜共患病和疾病潜力。结果表明，犬口腔内含有丰富多样的细菌群落，超过了以前基于培养和克隆的研究的估计。(C)2012 Elsevier B.V.保留所有权利。
Efficient characterization of the canine oral microbiome is critical for understanding the role of oral bacteria in health and for providing insight into early diagnosis and treatment strategies against periodontal disease. To date, characterization has been limited to cloning-based sequencing and conventional culture-based studies, which generally underestimate community diversity as a result of inherent biases in their methodologies. Pyrosequencing, a cloning- and culture-independent sequencing approach, eliminates these elements of bias from the analysis and enables extensive sequencing of microbial populations. In this report, pyrosequencing of the 16S rRNA gene was used to examine oral samples from six healthy dogs in an effort to determine community membership, diversity, and zoonotic implications. Pyrosequencing revealed a mean (SD) of 226 (59) operational taxonomic units (OTUs, 97% similarity), representing 181 genera from 13 bacterial phyla. The phyla Bacteroidetes (60.2%), Proteobacteria (20.8%), Firmicutes (11.4%), Fusobacteria (4.7%), and Spirochaetes (1.7%) predominated. The most commonly identified genera were Porphyromonas (39.2% of sequences), Fusobacterium (4.5%), Capnocytophaga (3.8%), Derxia (3.7%), Moraxella (3.3%), and Bergeyella (2.7%). Fifty-six OTUs, corresponding to 38 unique genus-level identifications, were present in all samples, which supports the concept of a stable core microbiome existing between healthy dogs. Potentially zoonotic and periodontal bacteria were detected in all dogs, and highlights the zoonotic and disease potential of the oral microflora. Results suggest that the canine oral cavity harbors a rich and diverse bacterial community, and exceeds estimates by previous culture- and cloning-based studies. (C) 2012 Elsevier B.V. All rights reserved.