Coproduction of Oligosaccharides and Glucose from Corncobs by Hydrothermal Processing and Enzymatic Hydrolysis
Coproduction of Oligosaccharides and Glucose from Corncobs by Hydrothermal Processing and Enzymatic Hydrolysis
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DOI：
10.1021/ie071201f
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发表时间：
2008
影响因子：
4.2
通讯作者：
G. Garrote;R. Yáñez;A. F. J. Alonso;J. Parajó
中科院分区：
文献类型：
--
作者：
G. Garrote;R. Yáñez;A. F. J. Alonso;J. Parajó
玉米芯在水条件下进行非等温反应，达到202 ~ 216℃的温度。分析了液的组成，以评估木聚糖转化为低聚糖和反应副产物，而处理后的废固体则进行酶水解。酶分析根据不完全、析因、中心实验设计进行，其中考虑了以下自变量：液固比（在6 - 16 g/g范围内），酶与底物比（在6 - 28滤纸单位/g范围内），β-葡萄糖苷酶活性/纤维素酶活性比（在1 - 9 IU/FPU范围内）。浓度/时间数据拟合到从文献中选择的方程中，模型参数表示为实验设计中考虑的操作变量的函数。在选定的条件下，获得了高的低聚糖产量和接近定量的纤维素转化为葡萄糖。
Corncobs were subjected to nonisothermal reaction in aqueous conditions to reach temperatures in the range 202−216 °C. The liquors were assayed for composition to assess the conversion of xylan into oligosaccharides and reaction byproducts, whereas the spent solids from treatments were subjected to enzymatic hydrolysis. Enzymatic assays were carried out according to an incomplete, factorial, centered design of experiments, in which the following independent variables were considered: liquor to solid ratio (in the range 6−16 g/g), enzyme to substrate ratio (in the range 6−28 filter paper units/g), and β-glucosidase activity/cellulase activity ratio (in the range 1−9 IU/FPU). The concentration/time data were fitted to an equation selected from the literature, and the model parameters were expressed as a function of the operational variables considered in the experimental design. Under selected conditions, high oligosaccharide yields and near quantitative cellulose conversion into glucose were obtained.