Differences between intestinal segments and soybean meal-induced changes in intestinal mucus composition of common carp Cyprinus carpio L.
Differences between intestinal segments and soybean meal-induced changes in intestinal mucus composition of common carp Cyprinus carpio L.
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DOI：
10.1111/anu.12041
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发表时间：
2014-02
影响因子：
3.5
通讯作者：
M. V. D. Marel;M. Pröpsting;F. Battermann;V. Jung-Schroers;A. Hubner;J. Rombout;D. Steinhagen
中科院分区：
文献类型：
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作者：
M. V. D. Marel;M. Pröpsting;F. Battermann;V. Jung-Schroers;A. Hubner;J. Rombout;D. Steinhagen
消化道是病原体和毒素可能进入的部位。除其他外，消化道受到粘液的保护。本研究采用组织学、组织化学和生物化学技术，对鲤鱼（Cyprinus carpio）肠道不同部位（从肠球到后肠）的组织样品和粗粘液制品进行了研究。此外，还观察了C.研究了鲤鱼对急性豆粕肠炎的保护作用。在本研究中，没有发现粘液高分子量糖蛋白（HMG）第一和第二段的不同蛋白质核心的迹象，但是可以发现粘液糖基化的差异。沿着肠轴，主要蛋白质峰的大小不相似，这可能是由不同的糖基化引起的。此外，发现抗菌肽β-防御素2的染色差异。此外，发现SBM饮食后HMG的变化与人类炎症性肠病（IBD）相似。最初的变化包括：粘蛋白组成的变化，BD 3和内脏中细菌的存在。在初始变化后，所有测量值均恢复至初始SBM前饮食值。
The alimentary tract is a possible site where pathogens and toxins can enter. The alimentary tract is protected, amongst others, by mucus. In this study, tissue samples and crude mucus preparations from different parts of the intestinal tract of Cyprinus carpio (from intestinal bulb onto the hindgut) were examined using histological, histochemical and biochemical techniques. Furthermore, the response of the intestinal mucosal layer and intestinal mucus of C. carpio to acute soybean meal (SBM)-induced enteritis was investigated. In the present study, an indication for a different protein core of mucus high molecular weight glycoproteins (HMGs) for first and second segment could not be found. However, differences in mucus glycosylation could be found. Along the gut axis, the size of the major protein peaks were not similar, which can be caused by a different glycosylation. Also, differences in staining for the antimicrobial peptide beta-defensin 2 were found. Furthermore, changes in HMGs upon SBM diet were found similar to those found in inflammatory bowel disease (IBD) in humans. Initial changes include: changes in mucin composition, the presence of BD3 and of bacteria in internal organs. After the initial changes, all values measured returned back to the initial pre-SBM diet values.