Lipocalin-type prostaglandin D synthase as a melanocyte marker regulated by MITF
Lipocalin-type prostaglandin D synthase as a melanocyte marker regulated by MITF
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DOI：
10.1016/j.bbrc.2005.11.125
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发表时间：
2006-01-27
影响因子：
3.1
通讯作者：
Shibahara, S
中科院分区：
文献类型：
--
作者：
Takeda, K;Yokoyama, S;Shibahara, S
小眼球相关转录因子(MITF)负责黑素细胞的分化。黑眼白色MITF(mi-BW)小鼠是一种隐性MITF突变体，由于皮肤和内耳中缺乏黑素细胞，其特征是毛色为白色和耳聋。通过基因芯片分析，我们已经发现了Lipocalin型前列腺素D合成酶(Lipocalin型前列腺素D合成酶，Lipocalin对野生型小鼠的免疫组织化学分析证实，L-PGDS在毛囊黑素细胞中特异表达。L-PGDS在B16小鼠黑色素瘤细胞中有表达，而在人黑色素瘤细胞系中检测不到。对MITF的干扰分析表明，L-PGDS在B16黑色素瘤细胞中的表达依赖于MITF。此外，我们还提供了MITF参与小鼠L-PGDS基因黑素细胞系特异性转录的证据。因此，L-PGDS代表了一个新发现的黑素细胞标志物。MITF可能通过激活黑素细胞中的L-PGDS基因来调节前列腺素D-2的产生。(C)2005 Elsevier Inc.保留所有权利。
Microphthalmia-associated transcription factor (MITF) is responsible for differentiation of melanocytes. A recessive MITF mutant, black-eyed white Mitf(mi-bw) Mouse, is characterized by white coat color and deafness, due to the lack of melanocytes in the skin and inner ears. By cDNA microarray analysis, we have identified lipocalin-type prostaglandin D synthase (L-PGDS), whose mRNA is undetectable in the homozygous Mitf(mi-bw) skin. Immunohistochemical analysis of wild-type mice identified the specific expression of L-PGDS in follicular melanocytes. L-PGDS mRNA is expressed in B16 mouse melanoma cells, but undetectable in human melanoma cell lines. RNA interference analysis against MITF suggests that L-PGDS expression is dependent on MITF in B 16 melanoma cells. Furthermore, we have provided evidence that MITF is involved in the melanocyte lineage-specific transcription of the mouse L-PGDS gene. Thus, L-PGDS represents a newly identified melanocyte marker. MITF may modulate the production of prostaglandin D-2 by activating the L-PGDS gene in melanocytes. (c) 2005 Elsevier Inc. All rights reserved.