Measuring Motivation Using the Progressive Ratio Task in Adolescent Mice.
Measuring Motivation Using the Progressive Ratio Task in Adolescent Mice.
复制标题
使用青春期小鼠的进步比率任务测量动机。
DOI：
10.1002/cpz1.776
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Canetta,Sarah
中科院分区：
文献类型：
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作者：
Cambre,Emily;Christenfeld,Elena;Torres,ArturoHerraez;Canetta,Sarah
寻求奖励的改变是多种精神疾病的标志，包括药物滥用和抑郁症。寻求奖励的一个重要方面是“想要”，这在人类和啮齿动物中都可以操作，比如在递进比任务中，需要越来越多的工作来获得给定的奖励。重要的是，许多具有寻求奖励缺陷的疾病被认为具有重要的神经发育成分，强调了能够研究整个生命周期中动机变化的重要性。虽然这项任务已经适用于成年和青少年大鼠，但在小鼠中，它主要用于测定成年小鼠的动机变化。将这项任务从成年小鼠调整到青春期小鼠的具体问题包括：(1)优化适合体重自然动态变化的生长动物的食物限制范式；(2)确定允许更年轻、更小的小鼠执行任务的任务条件，同时最大限度地减少在特定发育日期测量动机所需的行为塑造时间。为了实现这一目标，我们现在报告了一项对需要限制食物的发育中动物进行适当体重管理的方案，以及一项对青春期小鼠进行行为塑造和渐进比率测试的方案，包括评估动物在杠杆按压或鼻子戳作为必要的操作反应时表现更好。©2023 Wiley Periodicals llc .基本方案1：发育中小鼠的食物限制和体重管理备用方案：未预测生长图的发育中小鼠的食物限制和体重管理：基线小鼠的利用基本方案2：在青春期小鼠中进行操作箱设计、渐进式比率训练、测试和数据分析
Alterations in reward seeking are a hallmark of multiple psychiatric disorders, including substance abuse and depression. One important aspect of reward seeking is ‘wanting’, which can be operationalized in both humans and rodents in tasks such as the progressive ratio, in which an increasing amount of work is required to earn a given reward. Importantly, many disorders with reward‐seeking deficits are believed to have an important neurodevelopmental component, underscoring the importance of being able to study changes in motivation across the lifespan. Although this task has been adapted for both adult and adolescent rats, in mice it has predominantly been used to assay motivational changes in adults. Specific concerns in adapting this task from adult to adolescent mice include (1) optimizing a food restriction paradigm suitable for growing animals whose weights are naturally dynamically changing and (2) identifying task conditions that allow younger, smaller mice to perform the task while minimizing the length of the behavioral shaping required to measure motivation at specific developmental dates. Toward that end, we now report a protocol for appropriate weight management in developing animals that require food restriction, and a protocol for behavioral shaping and progressive ratio testing in adolescent mice, including an assessment of whether the animals perform better with lever presses or nose pokes as the required operant response. © 2023 Wiley Periodicals LLC.Basic Protocol 1: Food restriction and weight management in the context of developing miceAlternate Protocol: Food restriction and weight management of developing mice without projected growth chart: Utilization of baseline miceBasic Protocol 2: Operant box design, progressive ratio training, testing, and data analysis in adolescent mice