Radiation quality affects the efficiency of induction and the molecular spectrum of HPRT mutations in human T cells
Radiation quality affects the efficiency of induction and the molecular spectrum of HPRT mutations in human T cells
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DOI：
10.2307/3579720
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发表时间：
1997-11-01
影响因子：
3.4
通讯作者：
Jostes, R
中科院分区：
文献类型：
--
作者：
Albertini, RJ;Clark, LS;Jostes, R
Human T lymphocytes can be used to determine the frequency and molecular spectrum of somatic cell gene mutations induced by ionizing radiations both in vivo and in vitro. In vitro exposure of these G(0) cells to low-LET Cs-137 gamma rays results in the induction of HPRT mutations and a predominant molecular spectrum of DNA deletions and rearrangements, particularly total gene deletions (11-12%), Similar results are found in samples from humans exposed to low-LET radiation from I-131. The doubling dose for mutation induction is calculated to be 0.8 and 1.0 Gy from these exposures performed in vitro and in vivo, respectively, In vitro studies of the effects of high-LET radiation from exposure to Rn-222 also showed an induction of HPRT mutations, with a doubling dose of approximately 0.2 Gy. With this radiation, the predominant mutations were small partial deletions, with less than 2% total gene deletions. Studies of humans exposed to high-LET radiation from Pu-239 showed an increased HPRT mutant frequency for the group, although no significant dosimetry could be defined. In contrast to the humans exposed to I-131, no increase in the frequency of total gene deletions was found. This is consistent with the results for Rn-222 in vitro. The available data show that radiation quality affects both the efficiency of induction and the molecular spectrum of HPRT mutations in human T lymphocytes both in vitro and in vivo. The mutational spectrum may be relatively specific for radiations of different quality and thus allow a more precise measurement of the induction of somatic gene mutations resulting from individual exposures to radiation, and thereby provide more sensitive assessments of health risks. (C) 1997 by Radiation Research Society.
