SILAC-based quantitative proteomic analysis of secretome of Marc-145 cells infected with porcine reproductive and respiratory syndrome virus
SILAC-based quantitative proteomic analysis of secretome of Marc-145 cells infected with porcine reproductive and respiratory syndrome virus
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基于SILAC的猪繁殖与呼吸综合征病毒感染Marc-145细胞分泌组的定量蛋白质组学分析
DOI：
10.1002/pmic.201500486
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发表时间：
2016
期刊：
影响因子：
3.4
通讯作者：
Xiao Shaobo
中科院分区：
文献类型：
--
作者：
Zhao Fuwei;Fang Liurong;Wang Dang;Song Tao;Wang Ting;Xin Yinghao;Chen Huanchun;Xiao Shaobo
猪繁殖与呼吸综合征病毒（Porcine reproductive and respiratory syndrome virus，PRRSV）是猪繁殖与呼吸综合征（PRRS）的病原体，可引起母猪繁殖障碍，仔猪和生长猪呼吸道疾病，给全球养猪业造成巨大的经济损失。在本研究中，使用SILAC结合MS/MS来定量鉴定与模拟感染对照相比，PRRSV感染的Marc-145细胞中差异表达的分泌蛋白。总共，我们鉴定了204种分泌蛋白，其在感染细胞中显示出显著差异（163种上调，41种下调）。对分泌组数据的深入生物信息学分析表明，PRRSV感染强烈激活非经典蛋白质分泌，特别是囊泡介导的外泌体蛋白的释放，包括不同的危险相关分子模式分子和大多数参与蛋白质结合和转运、调节对刺激的反应、代谢过程和免疫反应的分泌蛋白。根据功能蛋白的分析，我们推测，蛋白质的结合，运输和免疫反应的PRRSV利用，以促进病毒复制和免疫逃避。我们的研究首次分析了PRRSV感染的Marc-145细胞的分泌蛋白谱，并为宿主对PRRSV感染的反应提供了有价值的见解。
Porcine reproductive and respiratory syndrome virus (PRRSV) is the causative agent of PRRS, which causes severe reproductive failure in sows, respiratory disease in young and growing pigs, and enormous economic losses to the global swine industry. In this study, SILAC combined with MS/MS was used to quantitatively identify the secretory proteins differentially expressed in PRRSV‐infected Marc‐145 cells compared with mock‐infected controls. In total, we identified 204 secretory proteins showing significant differences in infected cells (163 upregulated, 41 downregulated). Intensive bioinformatic analysis of secretome data revealed that PRRSV infection strongly activated nonclassical protein secretion, especially vesicle‐mediated release of exosomal proteins, including different danger‐associated molecular pattern molecules and the majority of secreted proteins involved in protein binding and transport, regulation of response to stimulus, metabolic processes, and immune responses. According to the functional proteins analysis, we speculate that proteins functioning in binding, transport, and the immune response are exploited by PRRSV to facilitate virus replication and immune evasion. Our study for the first time analyzes the secretory protein profile of PRRSV‐infected Marc‐145 cells and provides valuable insight into the host response to PRRSV infection.