Induction of cell apoptosis in 3T3-L1 pre-adipocytes by flavonoids is associated with their antioxidant activity
Induction of cell apoptosis in 3T3-L1 pre-adipocytes by flavonoids is associated with their antioxidant activity
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DOI：
10.1002/mnfr.200600040
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发表时间：
2006-11-01
影响因子：
5.2
通讯作者：
Yen, Gow-Chin
中科院分区：
文献类型：
--
作者：
Hsu, Chin-Lin;Yen, Gow-Chin
肥胖症在细胞水平上的生物学特征在于脂肪组织中从成纤维细胞前脂肪细胞分化的脂肪细胞的数量和大小增加。在这项研究中，我们专注于黄酮类化合物对细胞群体生长的影响与其抗氧化活性之间的关系。结果表明，黄酮类化合物（柚皮素、芦丁、橙皮苷、白藜芦醇、柚皮苷和槲皮素）对3 T3-L1前脂肪细胞的抑制率分别为28.3，8.1，11.1，33.2，5.6和71.5%。在氧自由基吸收能力（ORAC）测定中，槲皮素的ORACROO值最高。细胞凋亡实验表明，槲皮素以时间和剂量依赖的方式增加凋亡细胞。槲皮素处理的细胞降低线粒体膜电位的时间和剂量的过程中。细胞凋亡/坏死实验显示槲皮素可增加凋亡细胞的数量，但不增加坏死细胞的数量。槲皮素处理的细胞引起了一个显着的时间和剂量依赖性增加的caspase-3活性。Western分析表明，槲皮素处理显著下调PARP和Bcl-2蛋白，并激活caspase-3，Bax和巴克蛋白。这些结果表明，槲皮素有效抑制3 T3-L1前脂肪细胞中的细胞群体生长和细胞凋亡的诱导。
Obesity is biologically characterized at the cellular level by an increase in the number and size of adipocytes differentiated from fibroblastic pre-adipocytes in adipose tissue. In this study, we focused on the relationship between the influence of flavonoids on cell population growth and their antioxidant activity. The results showed that the inhibition of flavonoids (naringenin, rutin, hesperidin, resveratrol, naringin and quercetin) on 3T3-L1 pre-adipocytes was 28.3, 8.1, 11.1, 33.2, 5.6 and 71.5%, respectively. In oxygen radical absorbance capacity (ORAC) assay, quercetin had the highest ORACROO value among the six flavonoids tested. Apoptosis assays showed that quercetin increased apoptotic cells in time- and dose-dependent manner. Treatment of cells with quercetin decreased the mitochondrial membrane potential in the courses of time and dose. The cell apoptosis/necrosis assay showed that quercetin increased the number of apoptotic cells, but not necrotic cells. Quercetin treatment of cells caused a significant time- and dose-dependent increase in the caspase-3 activity. Western analysis indicated that treatment of querectin markedly down-regulated PARP and Bcl-2 proteins, and activated caspase-3, Bax, and Bak proteins. These results indicate that quercetin efficiently inhibits cell population growth and induction of apoptosis in 3T3-L1 pre-adipocytes.