DEMONSTRATION OF CELLS OF THE MONONUCLEAR PHAGOCYTE LINEAGE IN THE PERIODONTIUM FOLLOWING EXPERIMENTAL TOOTH MOVEMENT IN THE RAT - AN IMMUNOHISTOCHEMICAL STUDY USING MONOCLONAL-ANTIBODIES ED1 AND ED2 ON PARAFFIN-EMBEDDED TISSUES
DEMONSTRATION OF CELLS OF THE MONONUCLEAR PHAGOCYTE LINEAGE IN THE PERIODONTIUM FOLLOWING EXPERIMENTAL TOOTH MOVEMENT IN THE RAT - AN IMMUNOHISTOCHEMICAL STUDY USING MONOCLONAL-ANTIBODIES ED1 AND ED2 ON PARAFFIN-EMBEDDED TISSUES
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DOI：
10.1007/bf00572903
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发表时间：
1993-08-01
期刊：
影响因子：
--
通讯作者：
GOTZ, W
中科院分区：
文献类型：
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作者：
JAGER, A;RADLANSKI, RJ;GOTZ, W
在这项免疫组化研究中，两个单克隆抗体，ED 1和ED 2，它只识别单核吞噬细胞系统（MPS）在大鼠中的细胞，被施加到研究这些细胞的存在下，在牙周组织的重塑机械诱导正畸牙齿移动。免疫组化程序成功地进行了常规处理，石蜡包埋的组织切片，使用抗生物素蛋白-生物素-过氧化物酶复合物（ABC）技术。在大鼠脾脏和骨髓的阳性对照切片上可以显示MPS细胞。对于牙周组织重塑的研究，仅ED 1抗体被证明是合适的。使用该抗体，即使在对照动物中，也在整个牙周组织中观察到阳性单核细胞和多核细胞，即巨噬细胞和破骨细胞。诱导正畸牙移动后，巨噬细胞、破骨细胞和破牙细胞均被激活，对ED 1呈明显的阳性反应。
In this immunohistochemical study two monoclonal antibodies, ED1 and ED2, which recognize exclusively cells of the mononuclear phagocyte system (MPS) in the rat, were applied to study the presence of these cells during remodelling of the periodontal tissues following mechanically induced orthodontic tooth movement. The immunohistochemical procedure was carried out successfully on routinely processed, paraffin-embedded histological sections using the avidin-biotin-peroxidase-complex (ABC) technique. Cells of the MPS could be demonstrated on positive control sections of rat spleen and bone marrow. For the study of remodelling of the periodontal tissues only the ED1 antibody proved to be suitable. With this antibody, positive mononuclear and multinuclear cells, i.e. macrophages and osteoclasts, were seen throughout the periodontium even in the control animals. After the induction of orthodontic tooth movement activation of macrophages, osteoclasts and odontoclasts was demonstrable, all of them showing a clear-cut positive reaction to ED1.