Spatial control of branching within dendritic arbors by dynein-dependent transport of Rab5-endosomes
Spatial control of branching within dendritic arbors by dynein-dependent transport of Rab5-endosomes
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DOI：
10.1038/ncb1776
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发表时间：
2008-10-01
影响因子：
21.3
通讯作者：
Uemura, Tadashi
中科院分区：
文献类型：
--
作者：
Satoh, Daisuke;Sato, Daichi;Uemura, Tadashi
树突允许神经元整合感觉或突触输入，并且树突乔木内分支的空间布置和局部密度限制了输入的数量和类型（1，2）。黑腹果蝇树突状神经元为研究体内遗传程序提供了一个模型。在这里，我们报告的运动蛋白基因，包括动力蛋白亚基基因（dlic）和驱动蛋白重链（khc）的突变，不仅导致整体乔木的缩小，但也有一个显着的分支活动转移到近端区域内的乔木。当显性负性Rab5在突变神经元中表达时，这种表型被抑制，所述突变神经元在细胞体中沉积早期内体。我们还发现，1）在野生型神经元的树突分支中，含有Rab5的早期内体被动态转运，2）当Rab5单独功能被废除时，末端分支几乎完全缺失。这些结果揭示了微管马达和内体在树突形态发生中的重要联系。
Dendrites allow neurons to integrate sensory or synaptic inputs, and the spatial disposition and local density of branches within the dendritic arbor limit the number and type of inputs(1,2). Drosophila melanogaster dendritic arborization (da) neurons provide a model system to study the genetic programs underlying such geometry in vivo. Here we report that mutations of motor-protein genes, including a dynein subunit gene (dlic) and kinesin heavy chain (khc), caused not only downsizing of the overall arbor, but also a marked shift of branching activity to the proximal area within the arbor. this phenotype was suppressed when dominant-negative Rab5 was expressed in the mutant neurons, which deposited early endosomes in the cell body. We also showed that 1) in dendritic branches of the wild-type neurons, Rab5-containing early endosomes were dynamically transported and 2) when Rab5 function alone was abrogated, terminal branches were almost totally deleted. these results reveal an important link between microtubule motors and endosomes in dendrite morphogenesis.