Human-Scale Liver Harvest and Decellularization for Preclinical Research
Human-Scale Liver Harvest and Decellularization for Preclinical Research
复制标题
DOI：
10.1007/7651_2018_195
复制
发表时间：
2018-01-01
期刊：
影响因子：
--
通讯作者：
Kitagawa, Yuko
中科院分区：
文献类型：
--
作者：
Tajima, Kazuki;Yagi, Hiroshi;Kitagawa, Yuko
脱细胞支架在器官再生方面很有前景；为了便于对脱细胞的分析和临床前研究，使用大型动物的实验是必不可少的。一个使用大型动物的有效方案需要修改当前的方法，以考虑到解剖、大小、僵硬和动物处理方面的差异。本章描述了一种大型动物(特别是猪肝)肝脏脱细胞的方法，包括麻醉、器官采集、器官脱细胞和储存，并对潜在的缺陷进行了总结。这种方法可以假设地应用于大型动物的临床前研究和随后的临床应用。
Decellularized scaffolds are promising for organ regeneration; to facilitate analyses of decellularization and preclinical research, experiments using large animals are indispensable. An efficient protocol using large animals requires the modification of current methods to account for differences in anatomy, size, stiffness, and animal handling. This chapter describes a method for the decellularization of livers from large animals (especially porcine livers), including anesthesia, organ harvest, organ decellularization, and storage, with a summary of potential pitfalls. This methodology can hypothetically be applied to large animals for preclinical studies and subsequent clinical applications.