Expression pattern of EYA4 in the common marmoset (Callithrix jacchus) cochlea
Expression pattern of EYA4 in the common marmoset (Callithrix jacchus) cochlea
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DOI：
10.1016/j.neulet.2017.10.030
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发表时间：
2018-01-01
影响因子：
2.5
通讯作者：
Ogawa, Kaoru
中科院分区：
文献类型：
--
作者：
Matsuzaki, Saeko;Hosoya, Makoto;Ogawa, Kaoru
眼缺失（EYA）样基因是果蝇和哺乳动物感觉器官形成所必需的基因，Eya家族的EYA 4基因是人类迟发性中频感音神经性耳聋的致病基因，而Eya 4基因缺陷小鼠表现为先天性重度耳聋和咽鼓管畸形导致的渗出性中耳炎。由于EYA 4在人类耳蜗中的表型存在物种差异，其病理生理学机制尚未阐明。结果表明，EYA 4在成年绒猴耳蜗中有明显的表达模式，在所有支持细胞中表达都很强，而在小鼠中表达减弱。有趣的是，EYA 4在毛细胞、支持细胞和神经元中的表达与sine oculis同源框SIX1共定位，SIX1是EYA 4转录活性所必需的转录因子。结果表明，EYA 4基因在灵长类和啮齿类支持细胞中的表达模式存在种间差异。这些结果也表明EYA 4在灵长类听觉细胞中起着重要作用。用灵长类动物模型（如绒猴）或人类耳蜗细胞进行的实验可能会为EYA 4相关听力损失的未知发病机制提供线索。
The eyes absent (EYA)-like genes are essential for the formation of sensory organs among fly (Drosophila melanogaster) and mammals EYA4, one of the vertebrate genes of Eya family, is reported to be causative for late-onset mid-frequency sensorineural hearing loss in humans, while Eya4-deficient mice exhibited congenital profound deafness and otitis media with effusion due to the eustachian tube dysmorphology. Because of the species difference in the phenotype, the pathophysiology of EYA4 in the human cochlea has yet to be elucidated.Here, we examine the expression pattern of EYA4 in the cochlea of common marmoset (Callithrix jacchus), a non-human primate. The results indicated a distinct expression pattern of EYA4 in the adult marmoset cochleae, especially strong in all supporting cells, while in mouse their expressions were diminished. Interestingly, EYA4 expression in the hair cells, supporting cells and neurons was co-localized with sine oculis homeobox SIX1, a transcription factor essential for the transcriptional activity of EYA4. The results revealed inter-species differences in the expression pattern of EYA4 gene in supporting cells between primates and rodents. The results also indicated a fundamental role of EYA4 in the primate auditory cells. Experiments with primate models such as marmosets or with human cochlear cells may provide cues about the unknown pathogenesis of EYA4-related hearing loss.