Effects of optogenetic stimulation of vasopressinergic retinal afferents on suprachiasmatic neurones.
Effects of optogenetic stimulation of vasopressinergic retinal afferents on suprachiasmatic neurones.
复制标题
血管加压能视网膜传入的光遗传学刺激对视交叉上神经元的影响。
DOI：
10.1111/jne.12806
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发表时间：
2019
影响因子：
3.2
通讯作者：
Hume C
中科院分区：
文献类型：
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作者：
Hume C
生理昼夜节律是由下丘脑视交叉上核（SCN）协调的。SCN细胞的活动与环境信号同步，包括来自视网膜神经节细胞（RGC）的光信息。我们最近描述了一群表达加压素的RGCs（VP ‐ RGC），它们向SCN发送轴突投射。为了确定这些VP-RGC如何影响SCN中细胞的活性，我们使用光遗传学工具来特异性激活SCN内的轴突终末。向大鼠玻璃体内注射重组腺相关病毒，以在加压素启动子（VP-ChR2mCherry）下表达通道视紫红质-2和红色荧光蛋白mCherry。在急性脑切片中的体外记录显示，大约30%的腹外侧SCN细胞对光遗传学刺激作出反应，其放电率增加，在刺激的前200秒期间逐渐增加，并且在刺激结束后持续。最后，加压素V1A受体拮抗剂的应用抑制了对光遗传学刺激的反应。我们的数据表明，SCN内VP ‐ RGC轴突的光遗传学刺激以加压素依赖性方式影响SCN细胞的活性。
Physiological circadian rhythms are orchestrated by the hypothalamic suprachiasmatic nucleus (SCN). The activity of SCN cells is synchronised by environmental signals, including light information from retinal ganglion cells (RGCs). We recently described a population of vasopressin‐expressing RGCs (VP‐RGC) that send axonal projections to the SCN. To determine how these VP‐RGCs influence the activity of cells in the SCN, we used optogenetic tools to specifically activate their axon terminals within the SCN. Rats were intravitreally injected with a recombinant adeno‐associated virus to express the channelrhodopsin‐2 and the red fluorescent protein mCherry under the vasopressin promoter (VP‐ChR2mCherry). In vitro recordings in acute brain slices showed that approximately 30% of ventrolateral SCN cells responded to optogenetic stimulation with an increase in firing rate that progressively increased during the first 200 seconds of stimulation and which persisted after the end of stimulation. Finally, application of a vasopressin V1A receptor antagonist dampened the response to optogenetic stimulation. Our data suggest that optogenetic stimulation of VP‐RGC axons within the SCN influences the activity of SCN cells in a vasopressin‐dependent manner.