Generation of tooth-like structures from integration-free human urine induced pluripotent stem cells.
Generation of tooth-like structures from integration-free human urine induced pluripotent stem cells.
复制标题
利用免整合的人尿诱导多能干细胞生成齿状结构
DOI：
10.1186/2045-9769-2-6
复制
发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Pei D
中科院分区：
文献类型：
--
作者：
Cai J;Zhang Y;Liu P;Chen S;Wu X;Sun Y;Li A;Huang K;Luo R;Wang L;Liu Y;Zhou T;Wei S;Pan G;Pei D
牙齿不仅对一个好的微笑至关重要，而且对身体健康也至关重要。然而，我们经常因为意外或疾病而失去牙齿。解决这一问题的理想方法是用患者自身的细胞再生牙齿。在这里，我们描述了从无整合的人尿诱导多能干细胞（ifhU-iPSC）产生牙齿样结构。我们首先将ifhU-iPSC分化为上皮片，然后将其与E14.5小鼠牙齿间充质重组。在3周内从这些重组体中回收齿样结构，8种不同iPSC系的成功率高达30%，与H1 hESC相当。我们进一步检测到，ifhU-iPSC衍生的上皮片在牙齿样结构中分化成分泌釉质的成釉细胞，具有在正常人牙齿中发现的物理性质，如弹性模量和硬度。我们的研究结果表明，ifhU-iPSCs可用于再生患者特定的牙齿组织甚至牙齿，用于进一步的药物筛选或再生治疗。本文的在线版本（doi：10.1186/2045-9769-2-6）包含补充材料，可供授权用户使用。
Tooth is vital not only for a good smile, but also good health. Yet, we lose tooth regularly due to accidents or diseases. An ideal solution to this problem is to regenerate tooth with patients’ own cells. Here we describe the generation of tooth-like structures from integration-free human urine induced pluripotent stem cells (ifhU-iPSCs). We first differentiated ifhU-iPSCs to epithelial sheets, which were then recombined with E14.5 mouse dental mesenchymes. Tooth-like structures were recovered from these recombinants in 3 weeks with success rate up to 30% for 8 different iPSC lines, comparable to H1 hESC. We further detected that ifhU-iPSC derived epithelial sheets differentiated into enamel-secreting ameloblasts in the tooth-like structures, possessing physical properties such as elastic modulus and hardness found in the regular human tooth. Our results demonstrate that ifhU-iPSCs can be used to regenerate patient specific dental tissues or even tooth for further drug screening or regenerative therapies. The online version of this article (doi:10.1186/2045-9769-2-6) contains supplementary material, which is available to authorized users.