A library of 3342 useful nonamer primers for genome sequencing.
A library of 3342 useful nonamer primers for genome sequencing.
复制标题
包含 3342 个用于基因组测序的有用九聚物引物的文库。
DOI：
10.1016/0378-1119(90)90350-z
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发表时间：
1990
期刊：
影响因子：
3.5
通讯作者：
J. Slightom
中科院分区：
文献类型：
--
作者：
D. Siemieniak;J. Slightom
双链大重组DNA分子的引物定向测序由于寡脱氧核糖核苷酸引物合成中涉及的延迟和费用而尚未被接受。Studier [Proc. Natl. Acad. Sci. USA 86（1989）6917-6921]，用于使用短寡聚体引物文库。Szybalski [Gene 90（1990）177-178]提出了一种互补的建议，使用连接的六聚体来减少所需的寡聚体的数量。我们已经使用了一套规则的计算机辅助选择的图书馆组成的3342个特定的九聚体。使用GenBank中可获得的人类序列研究了该九聚体文库对特定基因测序的有效性。
Primer-directed sequencing, of double-stranded large recombinant DNA molecules, has not been accepted because of the delay and expenses involved in the synthesis of oligodeoxyribonucleotide primers. A potential solution to this problem was proposed by Studier [Proc. Natl. Acad. Sci. USA 86 (1989) 6917–6921] for using a library of short oligomer primers. Szybalski [Gene 90 (1990) 177–178] has made a complementary proposal using ligated hexamers to reduce the number of oligomers needed. We have used a set of rules for a computer-aided selection of a library consisting of 3342 specific nonamers. The effectiveness of this library of nonamers to sequence specific genes was studied using human sequences available in GenBank.