Caenorhabditis elegans PIEZO channel coordinates multiple reproductive tissues to govern ovulation
Caenorhabditis elegans PIEZO channel coordinates multiple reproductive tissues to govern ovulation
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DOI：
10.7554/elife.53603
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发表时间：
2020-06-03
期刊：
影响因子：
7.7
通讯作者：
Golden, Andy
中科院分区：
文献类型：
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作者：
Bai, Xiaofei;Bouffard, Jeff;Golden, Andy
PIEZO 1和PIEZO 2是新发现的机械敏感性离子通道，它们在响应机械刺激时表现出对钙的偏好。在这项研究中，我们发现了pezo-1，唯一的PIEZO直向同源物在Cajorhabditiselegans，在调节生殖的重要作用。一些缺失等位基因，以及一个假定的功能获得性突变体，PEZO-1造成了严重的减少育雏大小。体内观察表明，卵母细胞在排卵过程中进入和离开受精囊时会发生各种运输缺陷。排卵后卵母细胞在受精囊收缩过程中经常受到损伤。然而，在排卵期间，pezo-1突变体中的钙信号没有显著改变。PEZO-1的缺失也导致自体精子在排卵期间被推出受精囊后无法正确地导航回受精囊。这些发现表明PEZO-1在不同的生殖组织中起作用，以促进C.优雅的
PIEZO1 and PIEZO2 are newly identified mechanosensitive ion channels that exhibit a preference for calcium in response to mechanical stimuli. In this study, we discovered the vital roles of pezo-1, the sole PIEZO ortholog in Caenorhabditiselegans, in regulating reproduction. A number of deletion alleles, as well as a putative gain-of-function mutant, of PEZO-1 caused a severe reduction in brood size. In vivo observations showed that oocytes undergo a variety of transit defects as they enter and exit the spermatheca during ovulation. Post-ovulation oocytes were frequently damaged during spermathecal contraction. However, the calcium signaling was not dramatically changed in the pezo-1 mutants during ovulation. Loss of PEZO-1 also led to an inability of self-sperm to navigate back to the spermatheca properly after being pushed out of the spermatheca during ovulation. These findings suggest that PEZO-1 acts in different reproductive tissues to promote proper ovulation and fertilization in C. elegans.