TOPOGRAPHY OF CHOLINERGIC AND SUBSTANCE-P PATHWAYS IN THE HABENULO-INTERPEDUNCULAR SYSTEM OF THE RAT - AN IMMUNOCYTOCHEMICAL AND MICROCHEMICAL APPROACH
TOPOGRAPHY OF CHOLINERGIC AND SUBSTANCE-P PATHWAYS IN THE HABENULO-INTERPEDUNCULAR SYSTEM OF THE RAT - AN IMMUNOCYTOCHEMICAL AND MICROCHEMICAL APPROACH
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DOI：
10.1016/0306-4522(87)90337-x
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发表时间：
1987-04-01
期刊：
影响因子：
3.3
通讯作者：
FONNUM, F
中科院分区：
文献类型：
--
作者：
CONTESTABILE, A;VILLANI, L;FONNUM, F
在大鼠中研究了含胆碱能和P物质的缰核 - 脚间核投射的局部解剖结构。通过将胆碱乙酰转移酶和P物质免疫组织化学与对显微切割脑区的实验性损伤和生化分析相结合来开展研究。此外，为了对免疫组织化学数据进行量化，还进行了计算机辅助图像分析。结果表明，含胆碱能和P物质的神经元在缰内侧核中有不同的定位，并投射到脚间核基本不同的区域。胆碱能神经元聚集在缰内侧核腹侧的三分之二区域，而含P物质的细胞仅位于该核的背侧部分。在脚间核的大部分区域，含胆碱乙酰转移酶和P物质的纤维及终末也有类似的分隔，除了该核的最前端和最后端外，没有明显的重叠。胆碱能活性主要集中在核的中心核心区域，而P物质优先定位于脚间核的外周亚核。此外，P物质和胆碱乙酰转移酶的水平沿脚间核的头尾轴都显示出特殊的区域变化。实验性损伤的结果表明，由每一条缰核脚间束所携带的P物质投射在脚间核的吻侧部分主要是同侧的，并且随着到达核的更尾侧区域而逐渐变为双侧的。相比之下，由每一条缰核脚间束所携带的胆碱能投射混合得更快，并且在脚间核中仅显示出轻微的同侧优势。参考先前的解剖学和神经化学数据对研究结果进行了讨论，在一些情况下，这些先前的数据曾导致了不一致的解释。
The topography of cholinergic and substance P containing habenulo-interpeduncular projections has been studied in the rat. The research has been carried out by combining choline acetyltransferase and substance P immunohistochemistry to experimental lesions and biochemical assays in microdissected brain areas. In addition, computer-assisted image analysis has been performed in order to obtain quantification of immunohistochemical data. The results show that cholinergic and substance P containing neurons have a different localization in the medial habenula and project to essentially different areas of the interpeduncular nucleus. Cholinergic neurons are crowded in the ventral two-thirds of the medial habenula while substance P containing cells are exclusively localized in the dorsal part of the nucleus. In most parts of the interpeduncular nucleus, choline acetyltransferase and substance P containing fibres and terminals are similarly segregated and no overlapping is apparent except for the rostralmost and the caudalmost ends of the nucleus. Cholinergic activity is largely concentrated in the central core of the nucleus, while substance P is preferentially localized in the peripheral subnuclei of the interpeduncular nucleus. In addition, both substance P and choline acetyltransferase levels show peculiar regional variations along the rostrocaudal axis of the interpeduncular nucleus. The results of experimental lesions demonstrate that the substance P projection carried by each fasciculus retroflexus is prevailingly ipsilateral in the rostral part of the interpeduncular nucleus and becomes progressively bilateral as far as more caudal regions of the nucleus are reached. By contrast, the cholinergic projections carried by each fasciculus retroflexus intermingle more rapidly and only show a slightly ipsilateral dominance in the interpeduncular nucleus. The results of the study are discussed with reference to previous anatomical and neurochemical data which, in several instances, had given rise to discrepant interpretations.